Overexpression of PvWOX3a in switchgrass promotes stem development and increases plant height  

作　　者：Ruijuan Yang Zhenying Wu Chen Bai Zhichao Sun Mengqi Wang Yuzhu Huo Hailing Zhang Yamei Wang Huapeng Zhou Shaojun Dai Wenwen Liu Chunxiang Fu 

机构地区：[1]Shandong Provincial Key Laboratory of Energy Genetics and CAS Key Laboratory of Biofuels,Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences,266101 Qingdao,Shandong,China [2]University of Chinese Academy of Sciences,100049 Beijing,China [3]Shanghai Normal University,201418 Shanghai,China [4]Grass and Science Institute of Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang,China [5]Key Laboratory of Bio-resource and Eco-environment of Ministry of Education,College of Life Sciences,Sichuan University,610064 Chengdu,China

出　　处：《Horticulture Research》2021年第1期3506-3519,共14页园艺研究（英文）

基　　金：This work was supported by the Agricultural Variety Improvement Project of Shandong Province(No.2019LZGC010);National Natural Science Foundation of China(Nos.31672479,31872879,31801429,and 31701496);Youth Innovation Promotion Association of CAS(2019213);QIBEBT and Dalian National Laboratory for Clean Energy(DNL);CAS(Grant:QIBEBT I201921 QIBEBT I201930).

摘　　要：Switchgrass(Panicum virgatum L.)is an important perennial,noninvasive,tall ornamental grass that adds color and texture to gardens and landscapes.Moreover,switchgrass has been considered a forage and bioenergy crop because of its vigorous growth,low-input requirements,and broad geography.Here,we identified PvWOX3a from switchgrass,which encodes a WUSCHEL-related homeobox transcription factor.Transgenic overexpression of PvWOX3a in switchgrass increased stem length,internode diameter,and leaf blade length and width,all of which contributed to a 95%average increase in dry weight biomass compared with control plants.Yeast one-hybrid and transient dual-luciferase assays showed that PvWOX3a can repress the expression of gibberellin 2-oxidase and cytokinin oxidase/dehydrogenase through apparently direct interaction with their promoter sequences.These results suggested that overexpression of PvWOX3a could increase gibberellin and cytokinin levels in transgenic switchgrass plants,which promotes cell division,elongation,and vascular bundle development.We also overexpressed PvWOX3a in a transgenic miR156-overexpressing switchgrass line that characteristically exhibited more tillers,thinner internodes,and narrower leaf blades.Double transgenic switchgrass plants displayed significant increases in internode length and diameter,leaf blade width,and plant height but retained a tiller number comparable to that of plants expressing miR156 alone.Ultimately,the double transgenic switchgrass plants produced 174%more dry-weight biomass and 162%more solubilized sugars on average than control plants.These findings indicated that PvWOX3a is a viable potential genetic target for engineering improved shoot architecture and biomass yield of horticulture,fodder,and biofuel crops.

关 键 词：EXPRESSING BLADE landscape 

分 类 号：Q94[生物学—植物学]