喉鳞状细胞癌中α-烯醇化酶蛋白的表达及其与人乳头瘤病毒感染的关系  

Expression ofα-enolase protein and its relationship with infection with HPV16/18 in laryngeal carcinoma patients

作　　者：魏炜[1] 武月章 宋韫韬[1] 徐国辉[1] 石琦[2] Wei Wei;Wu Yuezhang;Song Yuntao;Xu Guohui;Shi Qi(Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education),Department of Head and Neck Surgery,Peking University Cancer Hospital&Institute,Beijing 100142,China;State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]北京大学肿瘤医院暨北京市肿瘤防治研究所,头颈外科恶性肿瘤发病机制及转化研究教育部重点实验室,100042 [2]中国疾病预防控制中心病毒病预防控制所,传染病预防控制国家重点实验室,北京102206

出　　处：《中华实验和临床病毒学杂志》2021年第6期626-630,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：传染病预防控制国家重点实验室杰青申报资助(2019SKLID501)。

摘　　要：目的观察α-烯醇化酶(α-enolase)蛋白在喉鳞状细胞癌中的表达、临床意义以及与人乳头瘤病毒(human papillomavirus,HPV)感染的关系。方法采用免疫组织化学方法检测98例病例α-enolase蛋白的表达并分析其与临床分期之间的关系;采用PCR、测序以及免疫组织化学方法检测病例标本中HPV16/18的表达情况,并分析其与α-enolase蛋白表达之间的关系。结果98例病例中免疫组织化学结果显示α-enolase蛋白的总阳性率为89.8%,HPV基因及蛋白检测总阳性率达到40.8%,纳入病例随着临床Ⅰ期到Ⅳ期的逐渐发展以及HPV16/18的感染,α-enolase蛋白的定位出现由胞质和胞核表达逐渐转移至更多的仅在胞质表达的倾向。结论α-enolase蛋白的表达随着病例临床病程的进展以及HPV16/18的感染,呈现了逐渐向胞质定位的趋势,α-enolase蛋白的检测可为喉癌的早期诊断和治疗提供实验室数据的参考。Objective To observe the expression ofα-enolase in laryngeal carcinoma patients and its relationship with the infection with HPV16/18.Methods The expression ofα-enolase protein in 98 cases was detected by immunohistochemical method and the relationship betweenα-enolase and clinical stage was analyzed.The expression of HPV16/18 was detected by PCR,sequencing and immunohistochemistry,the relationship between HPV16/18 expression andα-enolase expression was analyzed.Results Immunohistochemical result showed that the total positive rate ofα-enolase expression was 89.8%,and the total positive rate of HPV gene and protein detection was 40.8%.The localization ofα-enolase protein tended to shift from cytoplasm and nucleus expression to more cytoplasmic expression only.Conclusions With the progression of clinical course and HPV16/18 infection,the expression ofα-enolase protein showed a tendency of localization to the cytoplasm.Detection ofα-enolase protein can provide laboratory data reference for early diagnosis and treatment of laryngeal carcinoma.

关 键 词：喉癌 α-烯醇化酶 人乳头瘤病毒 免疫组织化学 

分 类 号：R739.65[医药卫生—肿瘤]