维生素C对创伤性脑损伤后神经细胞焦亡及NLRP3通路的影响  

Effects of Vitamin C on Nerve Cell Pyrolysis and NLRP3 Pathway Following with Traumatic Brain Injury

作　　者：陈燕伟 武秀权 王利[1] 李侠[1] 林伟[1] 蒋晓帆 张磊[1] CHEN Yan-wei;WU Xiu-quan;WANG Li;LI Xia;LIN Wei;JIANG Xiao-fan;ZHANG Lei(Department of Neurosurgery,Xijing Hospital,Air Force Medical University of PLA,Xi'an,Shaanxi,710032,China)

机构地区：[1]中国人民解放军空军军医大学西京医院神经外科,陕西西安710032

出　　处：《现代生物医学进展》2021年第24期4632-4636,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金面上项目(81974188);陕西省国际科技合作计划项目(2019KW-039);陕西省重点研发计划项目(2018SF-154)。

摘　　要：目的:考察创伤性脑损伤(TBI)中维生素C(Vit C)预处理抗神经细胞焦亡的作用和分子机制。方法:培养HT22细胞系,随机分为空白组(Sham组)、DMSO组(Con组)和Vit C预处理组(Vit C组),Vit C组分为低剂量和高剂量2组。Vit C组和Con组进行预处理72 h后划伤12 h,制成神经细胞创伤性脑损伤模型。利用荧光免疫组化法和Western-blot检测NLRP3的表达变化;标准ELISA试剂盒检测IL-1β和IL-18表达变化。结果:相较于Sham组,Con组和Vit C组中HT22细胞中NLRP3的染色阳性率高;与Con组比较,Vit C组的NLRP3表达明显减少(P<0.05),且IL-1β和IL-18表达也显著降低(P<0.05),各组间差异有统计学意义(P<0.05)。结论:TBI后利用不同剂量Vit C均可有效降低神经细胞焦亡,可能是通过下调NLRP3表达水平,减少IL-1β和IL-18等释放,发挥神经保护作用。Objective:To observed the effect and molecular mechanism of vitamin C(Vit C)pretreatment against nerve cell pyroptosis in traumatic brain injury(TBI).Methods:The HT22 cell lines were randomly divided into the sham group(sham group),DMEM control group(Con group)and the Vit C pretreatment group(Vit C group);Con group and Vit C group pretreatment in 72 h,then the TBI model was induced by scratch injury in the groups for 12 h and then sampling.The expression of NLRP3 was detected by immunofluorescence staining and Western-blot;The standard ELISA kit was used to detect the expression of IL-1βand IL-18.Results:Compared with the Sham group,the positive rate of NLRP3 staining in surviving neurons was high in the Con group and the Vit C groups;Compared with Con group,low and high dose Vit C can obviously reduce the expression level of NLRP3 protein,IL-1βand IL-18(P<0.05),and the difference among the low dose and the high dose groups was statistically significant(P<0.05).Conclusion:Different doses of Vit C effectively reduce nerve cell pyroptosis after TBI,possibly by alleviating the expression level of NLRP3,reducing the release of IL-1βand IL-18,and play important neuroprotective effects.

关 键 词：维生素C Nod样受体蛋白3 创伤性脑损伤 白介素-1Β 白介素-18 

分 类 号：R-33[医药卫生] R651.15