甲状腺乳头状癌组织SMYD2、GRK6表达与临床病理特征、增殖基因和预后的关系分析  

Analysis of the Relationship between SMYD2 and GRK6 Expression and Clinicopathological Features,Proliferation Genes and Prognosis in Papillary Thyroid Carcinoma

作　　者：董校良 王象征 王捷 方炜 宫扣飞 DONG Xiao-liang;WANG Xiang-zheng;WANG Ji;FANG Wei;GONG Kou-fei(General Surgery,Affiliated Hospital of Nanjing University of Traditional Chinese Medicine/Nanjing Integrated Traditional Chinese and Western Medicine Hospital,Nanjing,Jiangsu,210002,China;Emergency Surgery,Affiliated Hospital of Nanjing University of Traditional Chinese Medicine/Nanjing Integrated Traditional Chinese and Western Medicine Hospital,Nanjing,Jiangsu,210002,China;Cardio-Thoracic Surgery,Hefei Thnd People's Hospital Hefei,Anhui,230022,China;General Surgery,Puren Hospital of Hefei Economic Development Zone,Hefei,Anhui 230071,China;General Surgery,Hefei Eighth People's Hospital,Hefei,Anhui 230011,China)

机构地区：[1]南京中医药大学附属医院/南京市中西医结合医院普外科,江苏南京210002 [2]南京中医药大学附属医院/南京市中西医结合医院急诊外科,江苏南京210002 [3]合肥市第三人民医院心胸外科,安徽合肥230022 [4]合肥经开普仁医院普外科,安徽合肥230071 [5]合肥市第八人民医院普外科,安徽合肥230011

出　　处：《现代生物医学进展》2021年第24期4699-4704,共6页Progress in Modern Biomedicine

基　　金：江苏省南京市医学科技发展项目(YKK15159);江苏省自然科学基金项目(BK201602307)。

摘　　要：目的:探讨甲状腺乳头状癌组织中SET和MYND域蛋白2(SMYD2)、G蛋白偶联受体激酶6(GRK6)表达与临床病理特征、增殖基因和预后的关系。方法:选择2015年1月至2017年12月期间我院收治的83例甲状腺乳头状癌患者,取甲状腺乳头状癌患者手术切除的癌组织及癌旁组织(距肿瘤组织边缘≥5 cm)标本,采用qRT-PCR检测SMYD2、GRK6以及增殖基因[叉头盒蛋白A1(FOXA1)、程序性细胞死亡基因4(PDCD4)、Xklp2靶蛋白(TPX2)]表达。分析SMYD2、GRK6表达与临床病理特征、增殖基因和预后的关系。分析甲状腺乳头状癌患者预后的影响因素。结果:癌组织SMYD2、GRK6、FOXA1、PDCD4、TPX2表达高于癌旁组织(P<0.05)。Pearson相关性分析显示:SMYD2、GRK6表达与FOXA1、PDCD4、TPX2表达呈正相关(P<0.05)。肿瘤直径>1 cm、低中度分化、T3~T4a期、N1a~N1b期患者癌组织中SMYD2、GRK6表达高于肿瘤直径≤1 cm、高度分化、T1~T2期、N0期患者(P<0.05)。SMYD2、GRK6高表达组患者3年无进展生存期(PFS)、总生存期(OS)生存率低于SMYD2、GRK6低表达组(P<0.05)。Cox比例风险模型分析结果显示N1a~N1b期、SMYD2≥2.95、GRK6≥3.02是甲状腺乳头状癌患者不良预后的危险因素(P<0.05)。结论:甲状腺乳头状癌组织中SMYD2、GRK6表达增高,SMYD2、GRK6过表达与癌细胞增殖、侵袭和术后预后不良有关。Objective:To investigate the relationship between the SET and MYND domain-containing protein 2(SMYD2),G-protein coupled receptor kinase 6(GRK6)expression and clinicopathological features,proliferation genes and prognosis in papillary thyroid carcinoma tissues.Methods:A total of 83 patients with papillary thyroid carcinoma who were admitted to our hospital from January 2015 to December 2017 were selected.Surgically resected cancer tissues and paracancerous tissues(≥5 cm from the tumor tissue margin)of patients with papillary thyroid carcinoma were collected.The expression of SMYD2,GRK6 and proliferating proteins[forkhead box protein A1(FOXA1),programmed cell death gene 4(PDCD4),and target protein Xklp2(TPX2)]were detected by qRT-PCR.The relationship between the expression of SMYD2 and GRK6 and clinicopathological features,proliferative proteins and prognosis were analyzed.The prognostic factors of patients with papillary thyroid carcinoma were analyzed.Results:The expression of SMYD2,GRK6,FOXA1,PDCD4 and TPX2 in cancer tissues were higher than those in paracancerous tissues(P<0.05).Pearson correlation analysis showed that the expression of SMYD2 and GRK6 were positively correlated with the expression of FOXA1,PDCD4 and TPX2(P<0.05).The expression of SMYD2 and GRK6 in cancer tissues of patients with tumor diameter>1 cm,low and moderately differentiated,T3~T4 a stage and N1 a~N1 b stage were higher than those of patients with tumor diameter≤1 cm,highly differentiated,T1~T2 stage and N0 stage(P<0.05).The 3-year progression-free survival(PFS)and overall survival(OS)of patients with high SMYD2 and GRK6 expression groups were lower than those with low SMYD2 and GRK6 expression groups(P<0.05).Cox proportional risk model analysis showed that N1 a~N1 b stage,SMYD2≥2.95 and GRK6≥3.02 were risk factors for poor prognosis in patients with papillary thyroid carcinoma(P<0.05).Conclusion:The expression of SMYD2 and GRK6 in papillary thyroid carcinoma tissues are increased,and the overexpression of SMYD2 and GRK6 are rela

关 键 词：甲状腺乳头状癌 SMYD2 GRK6 临床病理特征 增殖基因 预后 

分 类 号：R736.1[医药卫生—肿瘤] R363[医药卫生—临床医学]