结直肠癌c⁃MET、CXCR4蛋白和微血管密度与肝转移的关系  

Correlation of c⁃MET and CXCR4 proteins and microvessel density with liver metastasis in colorectal cancer

作　　者：樊桂玲[1] 梁晓霞[1] 高宝红[1] 刘晓琴 白小利 王晞星[2] Fan Guiling;Liang Xiaoxia;Gao Baohong;Liu Xiaoqin;Bai Xiaoli;Wang Xixing(Department of Pathology,Shanxi Traditional Chinese Medical Hospital,Shanxi Provincial Academy of Traditional Chinese Medicine,Taiyuan 030012,China;Department of Oncology,Shanxi Traditional Chinese Medical Hospital,Shanxi Provincial Academy of Traditional Chinese Medicine,Taiyuan 030012,China)

机构地区：[1]山西省中医院山西省中医药研究院病理科,太原030012 [2]山西省中医院山西省中医药研究院肿瘤科,太原030012

出　　处：《肿瘤研究与临床》2021年第11期830-833,共4页Cancer Research and Clinic

基　　金：山西省重点研发计划(201903D321225);山西省中医药研究院院级基金(202010)。

摘　　要：目的探讨结直肠癌组织中c⁃MET、CXCR4蛋白和微血管密度(MVD)与结直肠癌肝转移的关系。方法收集2015年3月至2020年12月于山西省中医院手术切除的结直肠癌组织标本40例、癌旁(距肿瘤边缘5 cm)正常结直肠组织标本10例。40例结直肠癌患者中肝转移15例。采用免疫组织化学法检测各组织中c⁃MET、CXCR4蛋白及CD34标记的MVD情况,分析三者与肝转移间及三者间的关系。结果结直肠癌组织中c⁃MET蛋白阳性率[72.5%(29/40)比30.0%(3/10)]、CXCR4蛋白阳性率[47.5%(19/40)比10.0%(1/10)]及MVD(20.1±5.2比11.5±4.3)均高于癌旁组织,差异均有统计学意义(均P<0.05)。结直肠癌肝转移组中c⁃MET蛋白阳性率[86.7%(13/15)比64.0%(16/25)]和CXCR4蛋白阳性率[66.7%(10/15)比36.0%(9/25)]均高于无肝转移组,差异均有统计学意义(均P<0.05);结直肠癌肝转移组中MVD高于无肝转移组(21.5±5.3比12.4±5.7),差异有统计学意义(P<0.05)。在结直肠癌组织中,c⁃MET与CXCR4蛋白表达正相关(r=0.568,P<0.05),c⁃MET阳性及CXCR4阳性者MVD值均较阴性者高(均P<0.05)。结论c⁃MET蛋白、CXCR4蛋白及MVD在结直肠癌肝转移中可能起着重要作用,三个指标可为结直肠癌肝转移的早期诊断及预后预测提供一定的参考。Objective To explore the correlation of c⁃MET and CXCR4 proteins and microvessel density(MVD)with liver metastasis in colorectal cancer tissues.Methods A total of 40 colorectal cancer tissue samples and 10 paracancerous(5 cm from the edge of the tumor)normal colorectal tissue samples were collected from March 2015 to December 2020 in Shanxi Traditional Chinese Medical Hospital.Among 40 patients with colorectal cancer,15 patients had liver metastasis.Immunohistochemistry was used to detect c⁃MET protein,CXCR4 protein and CD34⁃labeled MVD in various tissues,and the relationships between them and liver metastasis and between the three were analyzed.Results The positive rates of c⁃MET protein[72.5%(29/40)vs.30.0%(3/10)],CXCR4 protein[47.5%(19/40)vs.10.0%(1/10)]and MVD(20.1±5.2 vs.11.5±4.3)in colorectal cancer tissues were higher than those in paracancerous tissues,and the differences were statistically significant(all P<0.05).The positive rates of c⁃MET protein[86.7%(13/15)vs.64.0%(16/25)]and CXCR4 protein[66.7%(10/15)vs.36.0%(9/25)]in colorectal cancer liver metastasis group were significantly higher than those in non⁃liver metastasis group,and the differences were statistically significant(both P<0.05).MVD in colorectal cancer liver metastasis group was significantly higher than that in non⁃liver metastasis group(21.5±5.3 vs.12.4±5.7),and the difference was statistically significant(P<0.05).In colorectal cancer tissues,c⁃MET protein expression was positively correlated with CXCR4 protein expression(r=0.568,P<0.05),and MVD in c⁃MET⁃positive patients or CXCR4⁃positive patients was higher than that in negative ones(both P<0.05).Conclusions The c⁃MET protein,CXCR4 protein and MVD may play important roles in the liver metastasis of colorectal cancer.The three indicators can provide a certain reference for the early diagnosis and prognosis prediction of liver metastasis of colorectal cancer.

关 键 词：结直肠肿瘤 肿瘤转移 肝肿瘤 原癌基因蛋白质c⁃met 受体 CXCR4 微血管 

分 类 号：R735.34[医药卫生—肿瘤]