白细胞介素10对高糖环境下牙槽骨在正畸力作用下骨改建的影响  

Effects of interleukin-10 on alveolar bone remodeling under orthodontic force in a high glucose condition

作　　者：韩瑜 李文静[1] 吴洁 崔占琴[1] Han Yu;Li Wenjing;Wu Jie;Cui Zhanqin(The Second Hospital of Hebei Medical University,Shijiazhuang 050000,Hebei Province,China)

机构地区：[1]河北医科大学第二医院,河北省石家庄市050000

出　　处：《中国组织工程研究》2022年第26期4180-4185,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:在正畸矫治力的作用下牙周组织中各种细胞因子水平发生变化,促使牙槽骨压力侧骨吸收和张力侧骨形成。而糖尿病患者体内的高糖水平及其他炎症因子水平的变化使其骨的代谢表现为吸收大于形成的不平衡状态。目的:观察高糖环境下白细胞介素10与机械刺激对人牙周膜细胞中骨保护素、核因子κB受体活化因子配体表达的影响,探究白细胞介素10对糖尿病患者牙槽骨在正畸力作用下骨改建的影响。方法:组织块法培养人牙周膜细胞并鉴定。根据人牙周膜细胞所受机械刺激分为压力组(又分为A,B,C,D组)和张力组(又分为E,F,G,H组),两组中的4个小组按字母顺序分别用低糖型DMEM、低糖型DMEN+白细胞介素10、高糖型DMEM、高糖型DMEM+白细胞介素10培养基培养,在0,12,24,48,72 h 5个时点收集细胞培养上清。采用ELISA法检测各组细胞培养上清液中骨保护素、核因子κB受体活化因子配体蛋白的表达。结果与结论:①压力可使人牙周膜细胞骨保护素蛋白表达减少,核因子κB受体活化因子配体蛋白表达增多,骨保护素/核因子κB受体活化因子配体比值减小,高糖可促进此过程,白细胞介素10与高糖作用相反;张力可使人牙周膜细胞骨保护素蛋白表达增多,核因子κB受体活化因子配体蛋白表达减少,二者的比值升高;说明高糖抑制此过程,白细胞介素10与高糖作用相反;②结果提示,高糖环境可使机械刺激下的人牙周膜细胞中骨保护素表达减少,核因子κB受体活化因子配体表达增多,骨保护素/核因子κB受体活化因子配体比值减小,白细胞介素10可拮抗高糖的作用。BACKGROUND:Orthodontic treatment can change the levels of various cytokines in periodontal tissue,which promotes bone resorption on the tooth pressure side and bone formation on the tooth tension side.Changes in high glucose level and other inflammatory factor levels in diabetic patients induce an imbalance in bone metabolism where absorbed bone tissue is more than that formed.OBJECTIVE:To observe the effects of interleukin-10 and mechanical stimulation on the expressions of osteoprotegerin and receptor activator of nuclear factor kappa B ligand in human periodontal ligament cells under high glucose environment,and to explore the effect of interleukin-10 on bone remodeling of alveolar bone in diabetic patients under orthodontic force.METHODS:Human periodontal ligament cells were cultured by tissue explant method.According to the mechanical stimulation the cells received,the cells were divided into stress group(A,B,C,D subgroups)and tension group(E,F,G,H subgroups).Both stress and tension groups included four subgroups(lowglucose DMEM group,low-glucose DMEM group+interleukin-10 group,high-glucose DMEM group,high-glucose DMEM+interleukin-10 group).Cell supernatant was collected at 0,12,24,48,and 72 hours after culture.The expressions of osteoprotegerin and receptor activator of nuclear factor kappa B ligand in the supernatant were detected by ELISA.RESULTS AND CONCLUSION:Pressure could decrease the expression of osteoprotegerin and increase the expression of receptor activator of nuclear factor kappa B ligand in human periodontal ligament cells,and decreased their ratio.High glucose could promote this process,while the effect of interleukin-10 was opposite to that of high glucose.Tension could increase the expression of osteoprotegerin,decrease the expression of receptor activator of nuclear factor kappa B ligand in human periodontal ligament cells,and increase their ratio.High glucose could inhibit this process,and interleukin-10 had an effect that was opposite to high glucose.To conclude,high glucose environment

关 键 词：高糖环境 白细胞介素10 骨保护素 RANKL 人牙周膜细胞 

分 类 号：R783[医药卫生—口腔医学] R446[医药卫生—临床医学]