Determination of the Content of Gastrodin in Jingtianshenma Tablets by High Performance Liquid Chromatography  

作　　者：Kun TANG Hongzhen GUO Jie QIAO Bing LI 

机构地区：[1]College of Life Science, Langfang Normal University, Langfang 065000, China [2]Technology Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province, Langfang 065000, China [3]Edible and Medicinal Fungi Research and Development Center of Hebei Universities, Langfang 065000, China [4]Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China

出　　处：《Medicinal Plant》2021年第6期15-16,19,共3页药用植物：英文版

基　　金：School-level Scientific Research Fund of Langfang Normal University-Doctoral Research Startup Project(XBQ202032);Self Raised Project of Langfang Science and Technology Bureau(2019012001);the Project of Langfang Key Laboratory of Food Nutrition and Safety.

摘　　要：[Objectives]The research aimed to establish a high performance liquid chromatography method for the content determination of gastrodin in Jingtianshenma Tablets.[Methods]A phenomenex Luna C18(250 mm×4.6 mm,5μm)column was used;the mobile phase was acetonitrile-0.05%phosphoric acid solution(3∶97);the detection wavelength was 220 nm,and the column temperature was set at 25℃.[Results]Gastrodin showed a good linear relationship in the range of 0.0984-0.5904μg with the peak area,and regression equation was Y=2000000X-51999(r=0.9999).The limits of detection and quantification for gastrodin were 2.50 and 4.20 ng respectively,and the average recovery rate was 95.95%.[Conclusions]This method is sensitive,accurate and reproducible,with good linearity,and it is suitable for the content determination of gastrodin in health food Jingtianshenma Tablets.

关 键 词：Jingtianshenma Tablets GASTRODIN HPLC Content determination 

分 类 号：TG1[金属学及工艺—金属学]