Investigation of optimum discharge characteristics and chemical activity of AC driven air plasma jet and its anticancer effect  

作　　者：Sansan PENG Dehui XU Miao QI Rong LIU Xinying ZHANG Huaiyan ZHANG Bolun PANG Jin ZHANG Hao ZHANG Zhijie LIU Dingxin LIU 彭三三;许德晖;祁苗;刘蓉;张新颖;张怀延;庞波伦;章金;张浩;刘志杰;刘定新(State Key Laboratory of Electrical Insulation and Power Equipment,Centre for Plasma Biomedicine,Xi'an Jiaotong University,Xi'an 710049,People's Republic of China;The School of Life Science and Technology,Xi'an Jiaotong University,Xi'an 710049,People's Republic of China)

机构地区：[1]State Key Laboratory of Electrical Insulation and Power Equipment,Centre for Plasma Biomedicine,Xi'an Jiaotong University,Xi'an 710049,People's Republic of China [2]The School of Life Science and Technology,Xi'an Jiaotong University,Xi'an 710049,People's Republic of China

出　　处：《Plasma Science and Technology》2021年第12期63-73,共11页等离子体科学和技术（英文版）

基　　金：supported by National Natural Science Foundation of China(Nos.12075188,52077166 and 51837008);State Key Laboratory of Electrical Insulation and Power Equipment(No.EIPE20302).

摘　　要：In this study,we investigated the effects of the quartz tube diameter,air flow rate,and applied voltage on the characteristics of an air plasma jet to obtain the optimized discharge characteristics.The physicochemical properties and concentration of reactive oxygen and nitrogen species(RONS)in plasma-activated medium(PAM)were characterized to explore their chemical activity.Furthermore,we investigated the inactivation effect of air plasma jet on tumour cells and their corresponding inactivation mechanism.The results show that the tube diameter plays an important role in sustaining the voltage of the air plasma jet,and the gas flow rate affects the jet length and discharge intensity.Additionally,the air plasma jet discharge displays two modes,namely,ozone and nitrogen oxide modes at high and low gas flow rates,respectively.Increasing the voltage increases the concentration of reactive species and the length of discharge.By evaluating the viability of A549 cells under different parameters,the optimal treatment conditions were determined to be a quartz tube diameter of 4 mm,gas flow rate of 0.5 SLM,and voltage of 18 k V.Furthermore,an air plasma jet under the optimized conditions effectively enhanced the chemical activity in PAM and produced more aqueous RONS.The air plasma jet induced significant cytotoxicity in A549 cancer cells after plasma treatment.H_(2)O_(2) and NO_(2) are regarded as key factors in promoting cell inactivation.The present study demonstrates the potential use of tumour cell therapy by atmospheric air PAM,which aids a better understanding of plasma liquid chemistry.

关 键 词：cold atmospheric plasma discharge characteristics reactive species plasma-activated medium anticancer effect 

分 类 号：R730.5[医药卫生—肿瘤] O539[医药卫生—临床医学]