Lipid droplets as endogenous intracellular microlenses  

作　　者：Xixi Chen Tianli Wu Zhiyong Gong Jinghui Guo Xiaoshuai Liu Yao Zhang Yuchao Li Pietro Ferraro Baojun Li 

机构地区：[1]Institute of Nanophotonics,Jinan University,511443 Guangzhou,China [2]Department of Physiology,School of Medicine,Jinan University,510632 Guangzhou,China [3]CNR-ISASI,Institute of Applied Sciences and Intelligent Systems«E.Caianiello»,Via Campi Flegrei 34,80078 Pozzuoli,Naples,Italy

出　　处：《Light(Science & Applications)》2021年第12期2507-2517,共11页光（科学与应用)（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(Nos.61905092,11874183,and 61827822);Guangdong Basic and Applied Basic Research Foundation(2021B1515020046);MIUR PRIN 2017–Morphological Biomarkers for early diagnosis in Oncology(MORFEO)Prot.2017N7R2CJ.

摘　　要：Using a single biological element as a photonic component with well-defined features has become a new intriguing paradigm in biophotonics.Here we show that endogenous lipid droplets in the mature adipose cells can behave as fully biocompatible microlenses to strengthen the ability of microscopic imaging as well as detecting intra-and extracellular signals.By the assistance of biolenses made of the lipid droplets,enhanced fluorescence imaging of cytoskeleton,lysosomes,and adenoviruses has been achieved.At the same time,we demonstrated that the required excitation power can be reduced by up to 73%.The lipidic microlenses are finely manipulated by optical tweezers in order to address targets and perform their real-time imaging inside the cells.An efficient detecting of fluorescence signal of cancer cells in extracellular fluid was accomplished due to the focusing effect of incident light by the lipid droplets.The lipid droplets acting as endogenous intracellular microlenses open the intriguing route for a multifunctional biocompatible optics tool for biosensing,endoscopic imaging,and single-cell diagnosis.

关 键 词：ENDOGENOUS LIPID OPTICS 

分 类 号：R318[医药卫生—生物医学工程]