哈蟆油药材中氨基酸含量与商品规格等级相关性研究  

Correlation between Amino Acid Content of Ranae Oviductus and Commodity Specifications

作　　者：朱丽婷 许亮[1] 张振秋[1] 李峰[1] 王姝婷 ZHU Liting;XU Liang;ZHANG Zhenqiu;LI Feng;WANG Shuting(Liaoning University of Traditional Chinese Medicine,Dalian 116600,Liaoning,China)

机构地区：[1]辽宁中医药大学,辽宁大连116600

出　　处：《中华中医药学刊》2021年第12期86-91,I0031,共7页Chinese Archives of Traditional Chinese Medicine

基　　金：中央本级重大增减支项目(2060302);辽宁省科技厅项目(2020-MS-224);辽宁省教育厅项目(L201942)。

摘　　要：目的根据不同批次哈蟆油药材中氨基酸的含量测定结果对不同商品规格等级的哈蟆油进行质量评价及等级区分。方法采用柱前衍生化-HPLC法同时测定不同规格等级哈蟆油中18种氨基酸的含量。应用系统聚类分析和主成分分析对不同商品规格等级哈蟆油进行类别划分;根据主成分分析结果计算不同批次样品的各主成分得分及综合得分。结果不同规格等级样品氨基酸总量均在69%~82%。通过主成分分析综合得分结果可以得出不同规格哈蟆油药材以氨基酸成分进行分析时线油的评分高于块油;不同等级的块油进行比较得出虽然一等块油中有两个得分较高,但总体来说二等块油的综合得分较一等块油高。结论应用该方法所测定氨基酸的含量可对不同商品规格等级的哈蟆油进行质量评价及等级区分。Objective According to the amino acid content,the quality evaluation and grade distinction of the different commodity specification grades of Ranae Oviductus were carried out.Methods The content of 18 amino acids in different specification grades of Ranae Oviductus was determined by RP-HPLC method.The classification of different commodity specification grades of Ranae Oviductus was divided by systematic clustering analysis method and main component analysis method,and the multiple main component scores and comprehensive scores of different batch samples were calculated according to the main component analysis results.Results The total amino acids in different batches of different specification grades were 69%~82%.Through the comprehensive scoring results of the main component analysis,it can be concluded that the score of line-type Ranae Oviductus was higher than that of block-type Ranae Oviductus when the different specifications of Ranae Oviductus were analyzed with amino acid composition,and the comparison of block-type Ranae Oviductus of different grades showed that although two of the first-class block-type Ranae Oviductus scored higher and the overall comprehensive score of second-class block-type Ranae Oviductus was higher than that of first-class.Conclusion The content of amino acids measured by RP-HPLC method can be used to evaluate the quality and grade differentiation of Ranae Oviductus of different commodity specifications.

关 键 词：哈蟆油 氨基酸 柱前衍生化 商品规格等级 

分 类 号：R284.1[医药卫生—中药学]