斜纹夜蛾四龄幼虫暴食相关基因的鉴定和通路分析  

Identification and pathway analysis of genes related to binge eating in taro caterpillar Spodoptera litura 4th instar larvae

作　　者：张赞 彭莉舒 李坤 钱坤[1] 相栋[2] 何林[1] Zhang Zan;Peng Lishu;Li Kun;Qian Kun;Xiang Dong;He Lin(College of Plant Protection,Southwest University,Chongqing 400716,Sichuan Province,China;Institute of Vegetable,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa 850000,Tibet Autonomous Region,China)

机构地区：[1]西南大学植物保护学院,重庆400716 [2]西藏自治区农牧科学院蔬菜研究所,拉萨850000

出　　处：《植物保护学报》2021年第6期1281-1290,共10页Journal of Plant Protection

基　　金：国家自然科学基金(32001962);中央高校基本科研业务费专项资金(SWU118094);博士后出站留(来)渝资助基金(2018LY43);西藏自治区重点研发计划(XZ202101ZY0006N)。

摘　　要：为绿色高效防治斜纹夜蛾Spodoptera litura,采用高通量测序技术对斜纹夜蛾暴食前期(3龄期)和暴食初始期(4龄期)进行转录组测序,筛选暴食前期和暴食初始期斜纹夜蛾差异基因,并对其高表达基因的代谢通路进行KEGG富集分析,随机选择缬氨酸、亮氨酸和异亮氨酸降解通路的5个关键基因对其表达量进行测定,以验证转录组测序结果的正确性。结果显示,从转录组数据中共拼接了17045条unigenes,其中有876条是新发现的unigenes,16625条unigenes有注释信息;5个基因的实时荧光定量PCR结果与转录组测序分析结果一致,表明转录组分析结果可信;在暴食初始期高表达的基因主要为血淋巴脂多糖结合蛋白基因,暴食初始期高表达基因富集度最大的通路主要为支链氨基酸代谢通路,其次是脂肪酸降解通路,表明暴食初始期斜纹夜蛾在增强其消化系统功能和营养吸收能力的同时,也加强了其能量代谢,为幼虫获取更多的食物提供动力。For controlling the taro caterpillar Spodoptera litura via an environmentally friendly and efficient way,high-throughput sequencing technology was used to analyze the transcriptome data of S.litura before the gluttonous stage(3 rd instar)and at the beginning of the gluttonous stage(4 th instar),and to screen the differentially expressed binge-related genes in the two periods.The metabolic pathways of highly expressed genes were then annotated via KEGG enrichment analysis,and five key genes in the valine,leucine and isoleucine degradation pathways were randomly selected and determined expression for verification of transcriptome results.The results of bioinformatic analysis showed that a total of 17045 unigenes were sequenced in the transcriptome data,of which 876 unigenes were not detected in the genome,and a total of 16625 unigenes were successfully annotated.The real-time quantitative PCR results of five randomly selected genes were consistent with transcriptome results,indicating that the results of transcriptome analysis were credible.The genes that were highly expressed during the beginning of the gluttonous stage were mainly the hemolymph lipopolysaccharide binding protein genes.The pathways with the highest enrichment of highly expressed genes during the beginning of the gluttonous stage were branched-chain amino acid metabolism pathways,followed by the fatty acid degradation pathway,indicating S.litura enhanced the function of its digestive system and increased nutrient absorption capacity at the beginning of gluttonous stage.

关 键 词：斜纹夜蛾 幼虫 暴食期 转录组测序 代谢通路 实时荧光定量PCR 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]