Can Hybrid Synapse be Formed between Rat Spinal Motor Neurons and Major Pelvic Ganglion Neurons in vitro?  

作　　者：CHENG Shigang XIANG Xuan LV Zemin MAO Xiaowen YANG Xinghai 

机构地区：[1]Department of Surgery,Maternal and Child Health Hospital of Hubei Province,Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430070,Hubei,China

出　　处：《Wuhan University Journal of Natural Sciences》2021年第6期521-526,共6页武汉大学学报（自然科学英文版）

基　　金：Supported by Key Natural Scienceof Hubei Province(2013CFA069)。

摘　　要：The purpose of this study is to determine whether synapses can be formed between spinal motor neurons(SMNs)and major pelvic ganglion(MPG)neurons of a rat in vitro.The green fluorescent protein(GFP)-labelled MPG cells were cultured together with SMNs in a specific medium.The synaptic-like contacts established between SMNs and MPG neurons were studied in co-cultures using morphologic and immunocytochemistry approaches.Phase-contrast observation of co-cultures showed apparent SMNs-MPG neurons contacts as early as three or four days in vitro.We demonstrate some evidence of synaptic contacts between SMNs and MPG neurons in vitro by immunostaining with antibody directed against postsynaptic density protein 95(PSD-95).We describe the development process of a defined SMNs-MPG neurons co-culture system.The results suggest that the hybrid synapse formation that may occur between SMNs and MPG neurons in vitro played an essential role in the mechanisms of a regenerated bladder with an artificial somatic-autonomic reflex arc.

关 键 词：spinal motor neurons major pelvic ganglion CO-CULTURE SYNAPSE 

分 类 号：R694[医药卫生—泌尿科学]