姜黄素对脑出血模型大鼠认知功能以及海马区细胞凋亡的影响  

Effects of curcumin on cognitive function and hippocampal cell apoptosis in rats with cerebral hemorrhage

作　　者：徐鹏 叶芸 乔鹏 XU Peng;YE Yun;QIAO Peng(Department of Neurology,Tongliao Hospital,Tongliao 028000,China)

机构地区：[1]通辽市医院神经内三科,内蒙古通辽028000

出　　处：《中风与神经疾病杂志》2021年第12期1076-1080,共5页Journal of Apoplexy and Nervous Diseases

摘　　要：目的研究选用姜黄素作为活性因子,通过脑出血大鼠模型来评价姜黄素对脑出血大鼠模型的认知和海马区细胞凋亡作用。方法将C57BL/6雄性大鼠建立的自发性脑出血患病动物模型,分为阴性对照组(0.005%乙醇/生理盐水)、姜黄素组(120 mg/kg,480 mg/kg),采用Morris水迷宫实验评价每组大鼠空间学习和记忆能力;采用大鼠神经元凋亡形态学和TUNEL分析法相结合对大鼠海马神经元组织病理学以及细胞凋亡比率进行测定;大鼠脑组织RNA分离测定caspase-3 mRNA分析;建立PC12细胞模型采用Western blotting分析PC12细胞中Bcl-2和Bax蛋白的表达。结果Morris水迷宫试验证实与患病组大鼠相比,姜黄素处理的大鼠具有较短的脱逃潜伏期,此外,穿越平台次数、平台象限停留时间、平台象限的总距离的百分比显著增加。姜黄素能显著降低患病caspase-3 mRNA和Bax蛋白的表达,同时增加Bcl-2/Bax的比值和Bcl-2蛋白的表达。结论姜黄素能显著改善脑出血大鼠的认知功能,潜在作用机制可能通过抑制神经元凋亡信号通路发挥预防神经元损伤的作用。Objective Currently,drugs used to treat intracerebral hemorrhage can only relieve the symptoms of patients,but cannot delay the development of the disease or cure the disease.In order to find more effective and less toxic drugs or nutritional supplements against cerebral hemorrhage,curcumin was selected as the active factor in this study to establish a rat model of cerebral hemorrhage to evaluate the effect of curcumin on cognition and hippocampal cell apoptosis in the rat model of cerebral.Methods C57 BL/6 male rats were selected to establish the animal model of spontaneous intracerebral hemorrhage.They were divided into negative control group(0.005%ethanol/normal saline)and curcumin group(120 mg/kg,480 mg/kg),Morris water maze test was used to evaluate the spatial learning and memory ability of rats;Apoptotic ratio was determined by apoptosis morphology combined with TUNEL analysis;RNA isolation in rat brain tissue carried out to determine caspase-3 mRNA expression;Western blotting was used to analyze the expression of bcl-2 and Bax proteins in PC12 cells.Results Morris water maze test confirmed that the rats treated with curcumin had a shorter escape incubation period compared with the rats in the sick group.In addition,the percentage of crossing times,residence time and total distance in the platform quadrant significantly increased(P<0.05).Curcumin significantly decreased the expression of caspase-3 mRNA and Bax protein in diseased mice,and increased the ratio of bcl-2/Bax and the expression of bcl-2 protein.Conclusion Curcumin could significantly improve the cognitive function of rats with cerebral hemorrhage.The potential mechanism of action may play a role in preventing neuron injury by inhibiting the neuronal apoptosis signaling pathway.

关 键 词：脑出血 姜黄素 认知功能 细胞凋亡 

分 类 号：R743.34[医药卫生—神经病学与精神病学]