‘鄂马铃薯3号’对干旱胁迫早期响应的转录组分析  

Transcriptome Analysis of Early Response of’Emalingshu 3’to Drought Stress

作　　者：刘圣宣 程云霞 刘腾飞[1] 宋波涛[1] LIU Shengxuan;CHENG Yunxia;LIU Tengfei;SONG Botao(Key Laboratory of Potato Biology and Biotechnology,Ministry of Agriculture and Rural Affairs/Hubei Potato Engineering Technology Research Center/Huazhong Agricultural University,Wuhan,Hubei 430070,China;College of Plant Sciences,Tarim University,Alar,Xinjiang 843300,China)

机构地区：[1]农业农村部马铃薯生物学与生物技术重点实验室/湖北马铃薯工程技术研究中心/华中农业大学,湖北武汉430070 [2]塔里木大学植物科学学院,新疆阿拉尔843300

出　　处：《中国马铃薯》2021年第6期481-488,共8页Chinese Potato Journal

基　　金：国家自然科学基金青年科学基金项目(32101781);国家农业(马铃薯)产业技术体系岗位科学家项目(CARS09-P07);湖北省第三批现代农业产业技术体系项目(HBHZD-ZB-2020-005)。

摘　　要：马铃薯通常被认为是干旱敏感性作物,由全球气候变化引起的干旱频发严重威胁了马铃薯的可持续生产。破译马铃薯的干旱应答机制,解析马铃薯干旱应答的核心因子,将有助于破解因干旱导致的马铃薯种植限制。基于此,研究旨在通过分析栽培品种应对干旱胁迫的早期响应过程,获得马铃薯早期干旱应答的关键候选基因。将‘鄂马铃薯3号’试管苗通过快速干旱胁迫处理,可在6 h出现明显的干旱胁迫表型,含水量相较于对照前也显著降低。不同干旱处理时间点的转录组分析结果显示,差异基因数量随着处理时间的延长而增加。2个分别编码转录因子GATA和YABBY的基因在干旱处理6 h后表达量极显著降低,可能为马铃薯干旱调控的负向因子。3个胚胎发育晚期富集蛋白(Late embryogenesis abundant,LEA)基因的表达受到干旱胁迫的剧烈诱导可作为潜在的干旱胁迫Marker基因。脱落酸(Abscisic acid,ABA)信号途径中的蛋白激酶StSnRK2.4和StSnRK2.8以及转录因子StAREB2受到干旱诱导最为剧烈,可能是ABA信号途径上响应干旱胁迫过程中的重要组分。Potato is generally considered to be a drought-sensitive crop.Frequent droughts caused by global climate change have seriously threatened the sustainable productions of potato.Deciphering the potato drought response mechanism and analyzing the core factors will help solve the potato planting restrictions caused by drought.Based on this,the present study aimed to obtain key candidate genes for potato drought response by analyzing the early response process of a cultivar to drought stress.The potato’Emalingshu 3’in vitro plantlets were treated with rapid drought stress,and the obvious drought stress phenotype appeared within 6 h,in which the water content was also significantly lower than that in the control.Transcriptome analysis showed that the number of differentially expressed genes(DEGs)was increased with the extension of treatment time.The expression levels of the two genes encoding transcription factors GATA and YABBY were significantly reduced after 6 h of drought treatment,which might be negative factors for potato drought regulation.The mRNA abundances of the three late embryogenesis abundant(LEA)genes were drastically induced by drought stress and could be used as potential drought stress marker genes.The protein kinases StSnRK2.4 and StSnRK2.8,and the transcription factor StAREB2 in the abscisic acid(ABA)signaling pathway were most induced by drought.They might be important components in ABA signaling pathway in response to drought stress.

关 键 词：马铃薯 干旱胁迫 转录组分析 转录因子 ABA信号途径 

分 类 号：S532[农业科学—作物学]