短双歧杆菌对Aβ;导致的阿尔兹海默症小鼠肠道菌群及代谢物的影响  

Effects of Bifidobacterium breve strains on gut microbiota and metabolites in Aβ-injected mice

作　　者：朱广素 赵建新[1] 张灏[1] 陈卫[1] 王刚[1] ZHU Guangsu;ZHAO Jianxin;ZHANG Hao;CHEN Wei;WANG Gang(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学食品学院,江苏无锡214122

出　　处：《食品与发酵工业》2022年第3期70-77,共8页Food and Fermentation Industries

基　　金：国家自然科学基金面上项目(31972052)。

摘　　要：为分析2株不同来源的短双歧杆菌对脑部微注射Aβ;蛋白导致的阿尔兹海默症小鼠肠道菌群及其代谢物的影响,给小鼠海马区注射Aβ;蛋白建立阿尔兹海默症小鼠模型,连续灌胃6周2株不同来源的短双歧杆菌,收集小鼠粪便,采用Illumina MiSeq高通量测序技术分析菌群的多样性及物种组成,并采用GC-MS技术检测小鼠粪便中短链脂肪酸的含量。菌群多样性分析发现,Aβ;蛋白注射改变了小鼠的菌群多样性及物种结构,灌胃短双歧杆菌可一定程度上改善菌群紊乱。进一步在属水平和种水平分析各组菌群差异,发现灌胃短双歧杆菌MY显著提高了大鼠肠道中产短链脂肪酸菌Coprococcus spp.、Lactobacillus reuteri和Akkermansia muciniphila的相对丰度,且有效调控了肠道内3种短链脂肪酸的水平。短双歧杆菌MY可能通过调节肠道菌群及其代谢物短链脂肪酸的水平缓解小鼠的认知障碍。To evaluate the effects of Bifidobacterium breve from different sources on intestinal microflora and its metabolites in mice with Alzheimer’s diseases(AD)induced by Aβ;injection,an animal model by injecting intrahippocampal Aβ;to mice was established,and two B.breve strains were separately administered to mice for 6 week.The microbial diversity and composition were measured.The microbial diversity and composition were significantly changed after Aβ;injection.Notably,B.breve supplementation displayed various effects on the recovery of gut microbiota dysbiosis.Metagenomic analysis showed that B.breve MY administration dramatically increased the relative abundance of Coprococcus spp.,Lactobacillus reuteri and Akkermansia muciniphila at genus and species level.In addition,GC-MS analysis found that supplementation with MY markedly modulated the concentrations of short-chain fatty acids(SCFAs).B.breve MY may delay the progression of AD by modulating the gut microbiota and SCFAs.

关 键 词：短双歧杆菌 脑肠轴 短链脂肪酸 阿尔兹海默症 肠道菌群 

分 类 号：R749.16[医药卫生—神经病学与精神病学]