一株反硝化聚磷菌的筛选鉴定及脱氮除磷性能研究  

Study on Identification and Nitrogen-phosphorus Removal Characteristics of a Denitrifying Phosphorus Accumulating Bacterium

作　　者：罗俊 孙霞 刘扬[3] 张虎 LUO Jun;SUN Xia;LIU Yang;ZHANG Hu(Hunan Bestall Water Conservancy Construction,Co.Ltd,Changsha Hunan 410007,China)

机构地区：[1]湖南百舸水利建设股份有限公司,湖南长沙410007 [2]山东江河湿地生态研究院,山东济南271100 [3]中国水利水电科学研究院,北京100080

出　　处：《环境科学导刊》2022年第1期1-4,共4页Environmental Science Survey

基　　金：湖南省科技重大专项资助项目(2018SK1010)。

摘　　要：为了从土著环境中获得对富营养化水体具有良好脱氮除磷效果的反硝化聚磷菌,从洞庭湖采集样品,通过富集培养、BTB平板分离、富氮富磷培养基复筛等方法,筛选获得一株具有脱氮除磷性能的菌株,命名为DT4-2。结合形态特征和16S rRNA基因序列分析,鉴定该菌株为Gemmobacter lanyuensis。考察了DT4-2菌株对人工合成污水的脱氮除磷效果,结果显示:初始接种量2%时,菌体生长状况最好,在反应体系中作用8h除磷率可达96.7%,作用60h除氮率可达到83.3%,表明该菌株在富营养化水体脱氮除磷方面具有良好的应用前景。order to obtain highly-efficient denitrifying phosphorous-accumulating bacteria simultaneously removing nitrogen and phosphors,strains were isolated from the samples of Dongting Lake.One effective strain,named as DT4-2,was obtained by culturing with enrichment medium,then isolating with BTB plate,further screening with nitrogen and phosphorus rich medium.The strain was identified as Gemmobacterlanyuensisby morphological characteristics and 16S rRNA gene sequence analysis.The effect of the strain on removing nitrogen and phosphorus in artificial wastewater was investigated.The results showed that strain DT4-2 could grow best with 2%initial inoculum,and the TP removal rate could reach 96.7%after 8h incubation,the TN removal rate was 83.3%after 60h in the reaction system.Therefore,the strain DT4-2 has a promising application prospect in eutrophic water treatment.

关 键 词：反硝化聚磷菌 脱氮除磷 富营养化 芽殖杆菌属 

分 类 号：X17[环境科学与工程—环境科学]