缺血性脑卒中致脑室运动纤毛功能障碍诱发脑水肿  

Ischemic stroke causes dysfunction of ventricular motile cilia and induces cerebral edema

作　　者：赵玉洁 张玲 马兰 滕增光 贾金鑫 曹晓璐 Zhao Yujie;Zhang Ling;Ma Lan;Teng Zengguang;Jia Jinxin;Cao Xiaolu(Dept of Environmental Health and Occupational Medicine,School of Public Health,Wuhan University of Science and Technology,Wuhan 430081;Key Laboratory of Occupational Hazard Identification and Control in Hubei Province,School of Public Health,Wuhan University of Science and Technology,Wuhan 430081)

机构地区：[1]武汉科技大学医学院公共卫生学院环境卫生与职业医学教研室,武汉430081 [2]武汉科技大学医学院职业危害识别与控制湖北省重点实验室,武汉430081

出　　处：《安徽医科大学学报》2022年第1期47-52,共6页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81801307);武汉科技大学科技创新团队培育项目(编号:2018TDZ03);湖北省高等学校优秀中青年科技创新团队计划项目(编号:T2020003)。

摘　　要：目的探讨室管膜细胞上运动纤毛功能对缺血性脑卒脑水肿的影响。方法60只SD大鼠随机分为假手术组和模型组。线栓法构建缺血2 h再灌注24 h模型。Longa’s 5分法进行神经功能评分;2,3,5-氯化三苯基四氮唑(TTC)染色评价脑梗死体积;干湿重法测量脑含水量;苏木精-伊红(HE)染色和扫描电镜(SEM)观察运动纤毛的形态变化;Western blot检测运动纤毛相关蛋白SPAG6表达水平;免疫荧光法检测运动纤毛结构和SPAG6的表达分布。结果与假手术组相比,模型组的脑梗死体积、神经功能评分明显增加,脑水肿明显(P<0.05)。HE染色和SEM结果显示,假手术组脑组织结构紧密、规则,室管膜细胞核圆润饱满,运动纤毛排列整齐、结构完整;模型组脑组织结构松散,室管膜细胞核皱缩浓染,出现大量坏死细胞,运动纤毛数量和密度显著降低、排列杂乱。Western blot结果显示,模型组的SPAG6总蛋白量较假手术组升高,但结果差异无统计学意义。免疫荧光实验结果显示,相比于假手术组,模型组运动纤毛长度明显较短(P<0.05);模型组的SPAG6总荧光强度较假手术组增高,但结果差异无统计学意义;模型组室管膜细胞上SPAG6的平均荧光强度与假手术组相比升高,差异有统计学意义(P<0.05);脑缺血再灌注(CIS-R)损伤使纤毛上的SPAG6荧光表达量降低(P<0.05)。结论脑缺血再灌注下调室管膜细胞表面运动纤毛上的SPAG6表达,造成运动纤毛结构和功能障碍,进而继发脑水肿损伤。Objective To investigate the effect of motile cilia of ependymal cells on cerebral edema induced by ischemic stroke.Methods SD rats(n=60)were randomly divided into sham group and ischemia-reperfusion model group.In the model group,a 2 h reperfusion 24 h model of ischemia was constructed by the method of suture.The Longa′s-5 point method and the 2,3,5-triphenyltetrazolium chloride(TTC)staining were used to assess neurological function and the cerebral infarction volume,respectively.The dry and wet weight method was used to measure brain water content.The morphological changes of motile cilia were observed by HE staining and scanning electron microscope(SEM).The level of SPAG6 expressed in tissues with motile cilia was measured by Western blot.Immunofluorescence was used to detect the structure of motile cilia and the expression distribution of SPAG6 in brain.Results Compared with the sham group,the infarct volume and neurological function score of the model group increased significantly,and the brain edema was obvious(P<0.05).HE staining and SEM results showed that the brain tissue structure of the sham group was tight and regular and the ependymal cell nucleus was round and full.The motile cilia in sham group were arranged orderly and the structure was complete.The brain tissue structure of the model group was loose and the ependymal cell nucleus was wrinkled、condensed and dyed.A large number of necrotic cells were generated,and the structure of motile cilia was disordered.The number and density of motile cilia were significantly reduced,and the arrangement was disordered.Western blot results showed that the total protein content of SPAG6 in the model group was higher than that in the sham group,but the results were not statistically different.Immunofluorescence results showed that compared with the sham group,the length of motile cilia in the model group was significantly shorter(P<0.05).The fluorescence intensity of SPAG6 in the model group was higher than that in the sham group,but the results were not sta

关 键 词：脑缺血/再灌注 脑水肿 室管膜细胞 运动纤毛 SPAG6 

分 类 号：R743.3[医药卫生—神经病学与精神病学]