lncRNA PITPNA-AS1靶向miR-92a-3p/TCF21对卵巢癌OVCAR-3细胞增殖和侵袭的影响  

Effect of lncRNA PITPNA-AS1 targeting miR-92a-3p/TCF21 on the proliferation and invasion of ovarian cancer OVCAR-3 cells

作　　者：曾洁 曾友玲 张清 陈说 杨玉 马元学 Zeng Jie;Zeng Youling;Zhang Qing;Chen Shuo;Yang Yu;Ma Yuanxue(Dept of Gynecology, The Affiliated Wuhan Children′s Hospital of Tongji Medical College of Huazhong University of Science & Technology,Wuhan Maternal and Child Healthcare Hospital,Wuhan 433015)

机构地区：[1]华中科技大学同济医学院附属武汉儿童医院(武汉市妇幼保健院)妇科,武汉433015

出　　处：《安徽医科大学学报》2022年第1期125-130,共6页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81602266)。

摘　　要：目的探讨长链非编码RNA PITPNA-AS1在卵巢癌中的作用及其可能的分子机制。方法采用荧光实时定量聚合酶链式反应(qPCR)技术检测卵巢癌组织和对应的癌旁组织、卵巢癌细胞系和正常卵巢上皮细胞系中PITPNA-AS1的表达水平。将PITPNA-AS1表达最少的细胞系分为对照组和实验组,分别转染阴性对照质粒或PITPNA-AS1质粒。细胞计数实验(CCK-8)法和Transwell法检测细胞的增殖活性和侵袭能力。生物信息学方法预测和双荧光素酶活性报告基因实验验证PITPNA-AS1作用的分子机制。qPCR和Western blot检测PITPNA-AS1相互作用的基因表达。结果PITPNA-AS1在卵巢癌组织中表达低于癌旁组织(P<0.01)。PITPNA-AS1在卵巢癌细胞系中的表达水平均低于正常卵巢上皮细胞(P<0.05),OVCAR-3细胞表达最少(P<0.01)。与对照组比较,过表达PITPNA-AS1能抑制OVCAR-3细胞的增殖活力(P<0.05)和侵袭能力(P<0.01)。PITPNA-AS1与miR-92a-3p存在靶向关系(P<0.01),miR-92a-3p与转录因子21(TCF21)存在靶向关系(P<0.01)。过表达PITPNA-AS1导致OVCAR-3细胞中miR-92a-3p的表达下降(P<0.01),TCF21基因的表达增加(P<0.01)。结论PITPNA-AS1在卵巢癌组织和细胞系中低表达,PITPNA-AS1可靶向调控miR-92a-3p/TCF21抑制卵巢癌OVCAR-3细胞的增殖和侵袭。Objective To explore the role of long non-coding RNA PITPNA-AS1 in ovarian cancer and its possible molecular mechanism.Methods Fluorescence real-time quantitative polymerase chain reaction(qPCR)technology was used to detect the expression level of PITPNA-AS1 in ovarian cancer tissues and corresponding adjacent tissues,ovarian cancer cell lines and normal ovarian epithelial cell lines.The cell lines with the least expression of PITPNA-AS1 were divided into control group and experimental group,and transfected with negative control plasmid or PITPNA-AS1 plasmid respectively.Cell counting(CCK-8)method and Transwell method were used to detect cell proliferation activity and invasion ability.Bioinformatics methods and dual luciferase activity reporter gene experiments predicted and verified the molecular mechanism of PITPNA-AS1.qPCR and Western blot were used to detect the gene expression of PITPNA-AS1 interaction.Results The expression of PITPNA-AS1 in ovarian cancer tissues was lower than that in adjacent tissues(P<0.01).The expression level of PITPNA-AS1 in ovarian cancer cell lines was lower than that in normal ovarian epithelial cells(P<0.05),and the expression in OVCAR-3 cells was the least(P<0.01).Compared with the control group,overexpression of PITPNA-AS1 could inhibit the proliferation activity(P<0.05)and invasion ability(P<0.01)of OVCAR-3 cells.PITPNA-AS1 had a targeting relationship with miR-92a-3p(P<0.01),and miR-92a-3p had a targeting relationship with transcription factor 21(TCF21)(P<0.01).Overexpression of PITPNA-AS1 caused a decrease in the expression of miR-92a-3p in OVCAR-3 cells(P<0.01),and an increase in the expression of TCF21 gene(P<0.01).Conclusion PITPNA-AS1 is lowly expressed in ovarian cancer tissues and cell lines.PITPNA-AS1 can inhibit the proliferation and invasion of ovarian cancer OVCAR-3 cells through targeted regulation of miR-92a-3p/TCF21.

关 键 词：PITPNA-AS1 卵巢癌 miR-92a-3p 转录因子21 

分 类 号：R737.31[医药卫生—肿瘤]