异源表达菊芋HtHCT基因对受体植物木质素相关生理生化指标的影响  

Effect of Heterologous Expression of HtHCT Gene fromHelianthus tuberosus on Lignin-related Physiologicaland Biochemical Indicators of Recipient Plants

作　　者：张新业 苏彦苹 朱姝 王聪艳[1,2] 侯晓强 李文静[1,2,3] ZHANG Xinye;SU Yanping;ZHU Shu;WANG Congyan;HOU Xiaoqiang;LI Wenjing(College of Life Science,Langfang Normal University,Langfang Hebei 065000,China;Hebei Key Laboratory of Animal Diversity,Langfang Hebei 065000,China;Langfang Key Laboratory of Cell Engineering and Application,Langfang Hebei 065000,China)

机构地区：[1]廊坊师范学院生命科学学院,河北廊坊065000 [2]河北省动物多样性重点实验室,河北廊坊065000 [3]廊坊市细胞工程与应用研究重点实验室,河北廊坊065000

出　　处：《西北农业学报》2022年第1期63-71,共9页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：河北省高等学校科学技术研究项目(BJ2018044);廊坊市科技支撑计划项目(2019012003)。

摘　　要：羟基肉桂酰辅酶A:莽草酸/奎宁酸羟基肉桂酰转移酶(HCT)是木质素合成过程中的关键酶之一。本研究构建了菊芋HtHCT基因的植物表达载体pCAMBIA1390-HtHCT,分别对拟南芥和本氏烟进行遗传转化,并对转基因植株进行生理生化指标测定。PCR和RT-PCR结果表明,HtHCT基因已经插入到受体植物基因组中,并能进行转录;转基因植株生理生化指标检测表明,HtHCT基因能够调节拟南芥和本氏烟中木质素的含量及组成,并对受体材料的类黄酮合成亦有一定的影响。Hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyltransferase(HCT)is one ofthe key enzymes involved in lignin synthesis.In this study,the plant expression vector pCAM-BIA1390-HtHCT of HtHCT gene from Helianthus tuberosus was constructed,and then the recombi-nant expression vector was transformed into Arabidopsis thaliana and Nicotiana benthamiana,re-spectively.At last,physiological and biochemical indicators of transgenic lines and wild type weremeasured.The PCR and RT-PCR results indicated HtHCT gene had been integrated into the genomeof recipient material and transcripted successfully.The analysis of physiological and biochemical indi-cators results showed that HtHCT could regulate the lignin content and composition in Arabidopsisthaliana and Nicotiana benthamiana.In addition,the flavonoids content of recipient plants was alsoaffected by the introduction of HtHCT.

关 键 词：菊芋 HtHCT基因 木质素 类黄酮 功能研究 

分 类 号：Q782[生物学—分子生物学] Q789