阿萨尔基亚芽孢杆菌对大丽轮枝菌微菌核的抑制作用  

Inhibition Effect of Bacillus axarquiensis TUBP1 on MicrosclerotiaGermination of Verticillium dahliae Kleb

作　　者：高畅 刘桂敏 曾红 GAO Chang;LIU Guimin;ZENG Hong(Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps,College of Life Sciences,Tarim University,Alar Xinjiang 843300,China;College of Life Sciences,Tarim University,Alar Xinjiang 843300,China)

机构地区：[1]塔里木大学生命科学学院,新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆阿拉尔843300 [2]塔里木大学生命科学学院,新疆阿拉尔843300

出　　处：《西北农业学报》2022年第1期99-104,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(31560525);新疆塔里木大学校长基金(TDZKCQ2020001)。

摘　　要：旨在分析阿萨尔基亚芽孢杆菌(TUBP1)对大丽轮枝菌(犞犲狉狋犻犮犻犾犾犻狌犿犱犪犺犾犻犪犲)微菌核的抑制作用。通过菌核萌发法、共培养、盆栽试验和扫描电镜观察,明确阿萨尔基亚芽孢杆菌发酵液对微菌核萌发的影响。菌核萌发试验结果表明,大丽轮枝菌微菌核经过阿萨尔基亚芽孢杆菌原发酵液、5倍和10倍稀释发酵液处理72h后,微菌核萌发率分别为62.52%、70.10%、75.33%;共培养结果显示,施加原浓度发酵液后大丽轮枝菌微菌核形成减少,由菌核型转变为菌丝型,且原浓度发酵液抑菌率达到64.69%;扫描电镜观察,经阿萨尔基亚芽孢杆菌原浓度发酵液处理后,分生孢子及微菌核形成减少,且随培养时间延长菌丝发生膨胀、断裂,出现异型,大丽轮枝菌自身形态结构被破坏。盆栽试验结果表明,对照组和处理组微菌核萌发率分别为61.15%和37.33%。Inhibition effect of Bacillus a.carquiensis TUBP1 on microsclerotia germination of Verticil-lium dahliae were analyzed in this paper.Fermentation broth of B.a.carquiensis TUBP1 acting on mi-crosclerotia germination of V.dahliae were detected by microsclerotia germination method,pot ex-periment and scanning electron microscope.The results showed that after inoculation with B.a.carquiensis TUBP1 original fermentation broth for 72 hours,5-fold and 10-fold diluted fermentationbroth,the germination rates of microsclerotia were 62.52%,70.10%and 75.33%,respectively.Coculture showed that the microsclerotia formation of V.dahliae in the original concentration fermentation broth decreased and the inhibition rate reached to 64.69%.Besides,the type of V.dahliaechanged from sclerotium to mycelium.In a greenhouse study,the microsclerotia germination rates ofthe control group and the treatment group were 61.15%and 37.33%,respectively.Under treatmentwith the original fermentation broth,the microsclerotia germination was lower than that of the controlgroup.The experimental results prove that B.acarquiensis TUBP1 has a certain inhibitory effect onmicrosclerotia germination of V.dahliae.

关 键 词：微菌核 阿萨尔基亚芽孢杆菌 抑制率 扫描电镜 

分 类 号：Q939.98[生物学—微生物学]