金磁微粒模拟酶比色检测谷胱甘肽的研究  

Detection of glutathione using Fe_(3)O_(4)@Au nanocomposite with peroxidase mimicking activity

作　　者：关桦楠[1] 吴巧艳 陈彦宇 薛悦 彭勃 GUAN Hua-nan;WU Qiao-yan;CHEN Yan-yu;XUE Yue;PENG Bo(College of Food Engineering,Harbin University of Commerce,Harbin 150028,China)

机构地区：[1]哈尔滨商业大学食品工程学院,黑龙江哈尔滨150028

出　　处：《现代化工》2022年第1期242-247,共6页Modern Chemical Industry

基　　金：黑龙江省科技重大专项项目(2019ZX08B02);国家自然科学基金项目(31871747,31201376,31370649);黑龙江省自然科学基金(C2016034);哈尔滨商业大学“青年创新人才”支持计划(2019CX05);黑龙江省博士后科研启动项目(LBH-Q19027);国家级大学生创新创业训练计划项目(202010240007)。

摘　　要：通过自组装法制备磁性纳米复合材料金磁微粒(Fe_(3)O_(4)@Au),对其进行表征,并优化Fe_(3)O_(4)@Au模拟酶检测谷胱甘肽的体系,确定检测限、回收率和抗干扰性。研究结果表明,最佳催化体系为:H_(2)O_(2)浓度50 mmol/L,催化温度60℃,反应时间为4 min。谷胱甘肽浓度在0.01~0.5 mmol/L时,吸光度差值与浓度呈现良好的线性关系,线性方程y=1.1575x+0.2728,线性相关系数R^(2)为0.9907,最低检测限度为0.3μmol/L,样品回收率分别为91.92%和110.31%,选择性良好。Fe_(3)O_(4)@Au nanocomposite is prepared by self-assembling method and characterized.Fe_(3)O_(4)Au mimetic enzyme system for detecting glutathione is optimized to determine the detection limit,recovery and anti-interference.The optimum detection conditions are obtained as follows:H_(2)O_(2) concentration is 50 mmol·L^(-1),catalytic temperature is at 60℃,and reaction time is 4 min.Under the optimized conditions,a good linear relationship is shown between the absorbance difference and the concentration of glutathione in the range of 0.01-0.5 mmol·L^(-1),and the equation is y=1.1575x+0.2728(R^(2)=0.9907).The lowest detection limit is 0.3μmol·L^(-1).The recoveries ranges from 91.92%and 110.31%.

关 键 词：金磁微粒 模拟酶 谷胱甘肽 食品分析 

分 类 号：O657.3[理学—分析化学] TS207.51[理学—化学]