岗南水库沉积物好氧反硝化菌群落时空分布特征  

Spatial and Temporal Distribution of Aerobic Denitrification Bacterial Community in Sediments of Gangnan Reservoir

作　　者：张紫薇 陈召莹 张甜娜 周石磊 崔建升[1] 罗晓[1] ZHANG Zi-wei;CHEN Zhao-ying;ZHANG Tian-na;ZHOU Shi-lei;CUI Jian-sheng;LUO Xiao(Pollution Prevention Biotechnology Laboratory of Hebei Province,College of Environmental Sciences and Engineering,Hebei University of Science and Technology,Shijiazhuang 050018,China)

机构地区：[1]河北科技大学环境科学与工程学院,河北省污染防治生物技术实验室,石家庄050018

出　　处：《环境科学》2022年第1期314-328,共15页Environmental Science

基　　金：中国科学院饮用水科学与技术重点实验室专项(20K04KLDWST);国家自然科学基金项目(51909056);河北省高等学校科学技术研究青年基金项目(QN2021064);河北科技大学引进人才科研启动基金项目(1181278)。

摘　　要：为研究岗南水库沉积物中好氧反硝化菌群的时空分布特征,运用好氧反硝化功能基因(napA)高通量测序技术,进行了α-多样性分析、微生物群落组成分析、微生物群落差异分析以及微生物网络分析.结果表明,α-多样性分析显示Chao1指数、Observed species指数和Shannon指数秋冬季均高于春夏季,即秋冬季采样点丰富度和多样性均较高.空间分布上,过渡区采样点的菌群的Chao1指数、Observed species指数、Shannon指数和Simpson指数均高于入库区和主库区采样点,即过渡区采样点菌群丰富度和多样性最高.岗南水库沉积物的好氧反硝化菌群主要属于变形菌门(Protebacteria),第一优势纲为β-Proteobacteria纲,第一优势属为陶厄氏菌属(Thauera).韦恩图分析(Venn)表明岗南水库沉积物各采样点好氧反硝化菌群分布均存在明显的时空差异.从空间水平基于LEfSe分析得到的差异物种比在季节水平的多.随机森林分析筛选得到标志物种中,Comamonas在所有空间分组采样点贡献程度最高,Pectobacterium在所有季节分组采样点贡献程度最高.网络分析表明存在9个主要模块包含297个节点565个边,关键物种分析得到47个关键物种OTUs.主坐标分析(principal co-ordinates analysis)显示关键物种的空间分布差异显著(Adonis,P<0.001).综上,通过对岗南水库沉积物好氧反硝化菌群组成结构及分布规律的研究,为后期高效好氧反硝化菌分离鉴定提供了科学依据.In order to study the spatial and temporal distribution characteristics of aerobic denitrification bacteria in the sediments of Gangnan Reservoir, the aerobic denitrification bacterial community was analyzed using a MiSeq high-throughput sequencing technique based on the napA gene. Moreover, the composition, diversity, difference, and network analysis of the aerobic denitrification bacterial community were investigated. The results showed higher α-diversity(Chao1 index, Observed species index, and Shannon index) in autumn and winter than that in spring and summer;that is, the richness and diversity of sampling sites in autumn and winter were higher. In terms of spatial distribution, the Chao1 index, Observed species index, Shannon index, and Simpson index of the bacterial community in the sampling sites in the transition zone were higher than those in the sampling sites in the inflow area and the main reservoir area, indicating that the richness and diversity of the bacterial community in the sampling sites in the transition zone were the highest. The aerobic denitrifying bacteria in the sediments of Gangnan Reservoir mainly belong to Protebacteria. The first dominant class was β-Proteobacteria, and the first dominant genus was Thauera. A Venn diagram analysis indicated that the community of aerobic denitrification bacteria exhibited significant spatial and temporal differences. At the spatial level, there were higher numbers of different species based on LEfSe analysis than that at the seasonal level. Among the marker species screened by random forest analysis, Comamonas had the highest contribution in all spatial sampling sites, whereas Pectobacterium had the highest contribution in all seasonal sampling sites. Network analysis showed that there were nine modules, including 565 edges of 297 nodes;47 keystone species were obtained based on the indicator OTUs analysis and network analysis. The principal co-ordinates analysis revealed that keystone species in the spatial distribution exhibited significant differ

关 键 词：岗南水库 沉积物 好氧反硝化 网络分析 生物多样性分析 

分 类 号：X172[环境科学与工程—环境科学] X524