温度对ANAMMOX生物膜工艺的脱氮影响与菌群结构分析  

Effect of Temperature on ANAMMOX Process in Sequencing Batch Biofilm Reactors: Nitrogen Removal Performance and Bacterial Community

作　　者：吴珊 王淑雅 王芬[1] 季民[1] WU Shan;WANG Shu-ya;WANG Fen;JI Min(School of Environmental Science and Engineering,Tianjin University,Tianjin 300350,China;Ji'nan Urban Construction Group Co.,Ltd.,Ji'nan 250031,China)

机构地区：[1]天津大学环境科学与工程学院,天津300350 [2]济南城建集团有限公司,济南250031

出　　处：《环境科学》2022年第1期416-423,共8页Environmental Science

基　　金：天津市科技计划项目(19ZXSZSN00080)。

摘　　要：针对厌氧氨氧化(anaerobic ammonium oxidation, ANAMMOX)工艺常低温运行问题,分别采用无纺布与改性聚氨酯泡沫塑料填料序批式生物膜反应器(sequence batch biofilm reactor, SBBR)处理含氮污水,考察了从35℃降至15℃过程的脱氮效果.结果表明,在进水氮容积负荷(NLR)为110 mg·(L·d)^(-1)时,无纺布填料反应器的平均总氮(TN)去除率先由71.16%(35℃)提升至76.19%(30℃)后又降低至61.11%(15℃),而改性聚氨酯泡沫塑料填料的平均TN去除率先由78.71%(35℃)提升至81.75%(20℃)后又降低至75.68%(15℃).与无纺布填料相比,改性聚氨酯填料脱氮效果更稳定,可作为优选填料.对改性聚氨酯填料生物膜进行高通量测序,结果表明主要ANAMMOX菌属为Candidatus Kuenenia,相对丰度分别为8%(35℃)、5%(25℃)和16%(15℃),可见,低温条件下,Candidatus Kuenenia仍会增殖富集.降温显著影响了生物膜群落结构.For the ambient and low-temperature operation of the anaerobic ammonium oxidation(ANAMMOX) process, sequence batch biofilm reactors(SBBR) with non-woven fabric and modified polyurethane foam as carriers, respectively, were used to treat nitrogenous wastewater. Nitrogen removal performances of both reactors were investigated while the temperature decreased from 35℃ to 15℃. When the nitrogen loading rate(NLR) was 110 mg·(L·d)^(-1), the average total nitrogen(TN) removal rate of the non-woven fabric reactor increased from 71.16%(35℃) to 76.19%(30℃) and then decreased to 61.11%(15℃). The average TN removal rate of the modified polyurethane foam reactor increased from 78.71%(35℃) to 81.75%(20℃) and then decreased to 75.68%(15℃). Compared with the non-woven fabric, the modified polyurethane foam provided a more stable nitrogen removal performance and could be used as the preferred carrier. High-throughput sequencing of the modified polyurethane foam biofilm indicated that Candidatus Kuenenia was the dominant ANAMMOX genus, with relative abundances of 8%(35℃), 5%(25℃), and 16%(15℃). Candidatus Kuenenia still grew and enriched at low temperatures. The decrease in temperature significantly affected the biofilm microbial community structure.

关 键 词：厌氧氨氧化(ANAMMOX) 温度 改性聚氨酯泡沫塑料填料 无纺布填料 微生物群落 

分 类 号：X172[环境科学与工程—环境科学] X703