MicroRNA-939在胰腺癌进展中的作用及机制  

The effect and mechanism of microRNA-939 in the progression of pancreatic cancer

作　　者：刘雅歆[1] 隋亚那 赵建鑫 吴红玉[1] 王文文[1] LIU Yaxin;SUI Yana;ZHAO Jianxin;WU Hongyu;WANG Wenwen(Department of Ultrasound,Weifang Traditional Chinese Medicine Hospital,Weifang 261041,China;Department of Emergency,Weifang Traditional Chinese Medicine Hospital;Department of Osteoarthropathy,Weifang Traditional Chinese Medicine Hospital)

机构地区：[1]潍坊市中医院超声科,山东潍坊261041 [2]潍坊市中医院急诊科 [3]潍坊市中医院骨关节病科

出　　处：《潍坊医学院学报》2021年第6期447-452,共6页Acta Academiae Medicinae Weifang

基　　金：潍坊市科技发展计划项目资助课题(项目编号:2019YX016)。

摘　　要：目的阐明microRNA-939(miR-939)在胰腺癌进展中的作用及机制。方法 qPCR分析20对胰腺癌患者组织中miR-939和PIM2的表达水平。通过CCK-8实验、平板克隆形成实验和Transwell侵袭迁移实验研究miR-939在胰腺癌中的生物学行为。通过TargetScan预测miR-939的靶基因,并通过双荧光素酶活性测定、western blot和qPCR进行验证。采用western blot和qPCR分析上皮细胞向间充质细胞的转化(epithelial to mesenchymal transition, EMT)标记。结果 MiR-939模拟物过表达miR-939显著抑制PANC-1细胞的增殖、迁移和侵袭,抑制EMT过程,而抑制物在MIAPaCa-2细胞中沉默miR-939则得到相反的结果。结论 MiR-939/PIM2信号通路是胰腺癌治疗的新靶点。Objective To illustrate the role and mechanism of microRNA-939(miR-939) in pancreatic cancer.Methods qPCR was used to analyze the expression levels of miR-939 and the PIM2 were analyzed in 20 pairs of tissue from patients with pancreatic cancer.The biological roles of miR-939 in pancreatic cancer were performed via CCK-8 assay, colony formation assay and Transwell invasion and migration assays.Target genes of miR-939 were predicted by TargetScan and verified via a dual luciferase activity assay, western blot and qPCR.The EMT marker was analyzed by western blot and qPCR.Results Overexpression of miR-939 mimics significantly inhibited the proliferation, migration and invasion of PANC-1 cells, as well as the EMT process.However, miR-939 silencing in MIAPaCa-2 cells by inhibitors got the opposite results.Conclusion The findings of the present study demonstrated that miR-939/PIM2 axis may serve as a new therapeutic target for the treatment of pancreatic cancer.

关 键 词：胰腺肿瘤 MicroRNA-939 PIM2 侵袭和迁移 EMT 

分 类 号：R735.9[医药卫生—肿瘤]