蛋白质组学中样品鉴定及定量的影响因素概述  

Factors influencing sample identification and quantification in proteomics

作　　者：李丙旭 陈吴越 王姣姣 彭珊 吕翔[1] 马宏跃[1] 周婧[1] LI Bing-xu;CHEN Wu-yue;WANG Jiao-jiao;PENG Shan;Lü Xiang;MA Hong-yue;ZHOU Jing(College of Pharmacy,Nanjing University of Traditional Chinese Medicine,Nanjing 210023,China)

机构地区：[1]南京中医药大学药学院,南京210023

出　　处：《药物分析杂志》2021年第12期2049-2054,共6页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金(81673563,81274199,82073975);江苏省方剂高技术研究重点实验室/江苏省中药资源产业化过程协同创新中心资助项目(FJGJS-2015-15,ZDXM-14);江苏省青蓝工程学术带头人项目。

摘　　要：蛋白质是生物体结构和功能调节的关键组成成分,具有极高的复杂性。基于质谱的蛋白质组学分析技术已成为目前蛋白质鉴定和定量研究的主要方法,在许多疾病相关生物标志物的筛选及药物的靶点鉴定方面显示出巨大优势。本文概括了基于质谱的自下而上蛋白质组学分析方法,并详细介绍了该流程下样品制备、色谱分离、质谱数据采集模式以及参数设置等对蛋白质鉴定及定量的影响。旨在优化临床样品中蛋白质鉴定及定量结果的分析精度、准确度及覆盖率,为后续药物设计与发现提供参考。Protein is the key component in the regulation of biological structure and functions,which is of great complexity.Mass spectrometry(MS)-based proteomics has become the main method for protein qualitative and quantitative research and showed great advantages in the screening of many disease-related biomarkers and identification of drug targets.In this paper,the MS-based bottom-up proteomics analysis was briefly summarized,besides,the effects of sample preparation,liquid chromatographic separation,mass spectrum acquisition strategies and the setting of relative parameters on protein identification and quantification were introduced in detail.The purpose of this review is to optimize the precision,accuracy and coverage of protein qualitative and quantitative results in clinical samples and provide a reference for subsequent drug design and discovery.

关 键 词：质谱 蛋白质组学 鉴定 定量 影响因素 

分 类 号：R917[医药卫生—药物分析学]