壮骨止痛胶囊对去卵巢大鼠股骨组织Runx2、Osx及DKK1蛋白表达的影响  

Effect of Zhuanggu Zhitong Capsule on the Expression of Runx2,Osx and DKK1 Protein in Femur Tissues of Ovariectoid Rats

作　　者：王桂云 吴结枝 梁琼 雷晓明[1] 李荣慧 曹宝丹 刘慧萍[1] 张国民[1] 刘平安 Wang Guiyun;Wu Jiezhi;Liang Qiong;Lei Xiaoming;Li Ronghui;Cao Baodan;Liu Huiping;Zhang Guomin;Liu Pingan(College of Integrated Chinese and Western Medicine,Hunan University of Chinese medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学中西医结合学院,长沙410208

出　　处：《世界科学技术-中医药现代化》2021年第10期3621-3629,共9页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委员会面上项目(81973920):壮骨止痛方治疗去势大鼠骨质疏松症的联合组学网络调控机制及其生物力学效应的三维有限元研究,负责人:雷晓明;湖南省教育厅科学研究重点项目(2019A370):壮骨止痛方对去势大鼠骨髓间充质干细胞MAPK信号通路中ERK1/2和p38MAPK蛋白调控机制的研究,负责人:张国民;湖南省教育厅科学研究重点项目(2020A358):基于miR-322调控BMP-2/OSX研究壮骨止痛方对骨质疏松症的干预机制,负责人:刘平安。

摘　　要：目的观察壮骨止痛胶囊(Zhuang Gu Zhi Tong Capsule,ZGZTC)对去卵巢大鼠股骨组织Runx2(成骨细胞分化和软骨细胞成熟所必需的转录因子之一)、Ostrix(Osx或Sp7,成骨细胞分化所需的转录因子,能与基因启动子上的Sp结合位点结合并调节其表达)、Dickkopf-1(DKK1,一种分泌型Wnt拮抗剂)表达的影响,探讨其治疗绝经后骨质疏松的机制。方法按体质量将60只3月龄SPF级雌性大鼠随机分为6组:空白组(BlankGroup,B)、模型组(ModelGroup,M)、骨松宝组(Gusong bao Group,GSB)、壮骨止痛胶囊高剂量(ZGZTC-HD)、壮骨止痛胶囊中剂量(ZGZTC-MD)、壮骨止痛胶囊低剂量(ZGZTC-LD)组,每组10只。除空白组外,其余5组均采用双侧去卵巢法制备绝经后骨质疏松模型。术后1周开始药物干预,连续13周。取股骨,观察其病理形态及Runx2、Osx、DKK1蛋白的表达。结果与M相比较,GSB、ZGZTC-HD、ZGZTC-MDD骨小梁面积基本恢复,骨髓腔面积无明显扩大,梁髓比升高明显;与M相比,GSB、ZGZTC-HD、ZGZTC-MD、ZGZTC-LD四组大鼠股骨组织的Runx2蛋白和Osx蛋白表达率上升(P<0.05)、DKK1蛋白表达率下降(P<0.05)。结论ZGZTC可以调节Osx、Runx2及DKK1蛋白表达,Osx和Runx2蛋白在股骨组织中起正向调节作用,DKK1蛋白起负向调节作用,这可能是ZGZTC防治PMOP的作用机制之一。Objective To observe the effects of Zhuang Gu Zhi Tong Capsule(ZGZTC)on Runx2(one of the transcription factors necessary for osteoblast differentiation and chondrocyte maturation),Ostrix(Osx or Sp7,a transcription factor required for osteoblast differentiation,can bind to the Sp binding site on the gene promoter and regulate its expression),and the effect of Dickkopf-1(DKK1,a secreted Wnt antagonist)expression,and to explore its mechanism of treating postmenopausal osteoporosis.Methods According to body weight,60 female rats of SPF grade were divided into 6 groups:Blank Group(B),Model Group(M),Gusongbao Group(GSB),ZGZTC High Dose(ZGZTC-HD),ZGZTC Medium Dose(ZGZTC-MD),ZGZTC Low Dose(ZGZTC-LD)groups,with 10 rats in each group.Except for the blank group,in order to prepare PMOP models,the remaining 5 groups of rats all had their ovaries removed.Drug intervention was started one week after surgery for 13 consecutive weeks.And we took the femur and observed its pathological morphology and the expression of Runx2,Osx,and DKK1 protein.Results Compared with M,the trabecular bone area of GSB,ZGZTC-HD,ZGZTC-MD basically recovered,the bone marrow cavity area was not significantly enlarged,and the beam-to-medullary ratio increased significantly;Compared with M,GSB,ZGZTC-HD,ZGZTC-MD,ZGZTC-LD increased the expression rate of Runx2 protein and Osx protein in rat femur tissue(P<0.05),while the expression rate of DKK1 protein decreased(P<0.05).Conclusion ZGZTC can regulate the expression of Osx,Runx2 and DKK1 proteins.Osx and Runx2 proteins play a positive regulatory role in femoral tissue,while DKK1protein plays a negative regulatory role.This may be one of the mechanisms of ZGZTC in preventing and treating PMOP.

关 键 词：壮骨止痛胶囊 绝经后骨质疏松症 RUNX2 成骨细胞分化所需的转录因子Osterix DICKKOPF-1 

分 类 号：R285.5[医药卫生—中药学]