乳酸乳球菌Z-2不同处理方式对鲤益生作用的离体研究  

Probiotic Characteristics of Different Preparations for Lactococcus lactis Z-2 on Cyprinus carpio L.in Vitro

作　　者：蔡忠良 张静静 王淏冉 任欣欣 朱超杰 刘莎莎 崔文姗 冯军厂 CAI Zhongliang;ZHANG Jingjing;WANG Haoran;REN Xinxin;ZHU Caojie;LIU Shasha;CUI Wenshan;FENG Junchang(College of Fisheries,Henan Normal University,Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation,Engineering Lab of Henan Province for Aquatic Animal Disease Control,Henan Xinxiang 453007,China)

机构地区：[1]河南师范大学水产学院,河南省水产动物养殖工程技术研究中心,水产动物疾病控制河南省工程实验室,河南新乡453007

出　　处：《饲料工业》2022年第2期46-51,共6页Feed Industry

基　　金：河南省重点科技攻关项目[212102110106]。

摘　　要：为探究乳酸乳球菌Z-2(Lactococcus lactis Z-2)不同处理方式对鲤的益生作用,试验共设置L.lactis Z-2菌株6种不同处理[活菌及上清液的混合物(mixture of live cells and supernatant,LCS)、无细菌培养上清液(cell-free culture supernatant,CS)、活菌(live cells,LC)、菌热灭活(heat-killed cells,HK)、菌超声破碎(ultrasonic broken of cells,UB)和胞外多糖(exopolysaccharides of CS,EPS-2)]与鲤[体重(47.66±0.43)g]头肾单核细胞体外共培养,检测其对鲤头肾单核细胞的增殖能力和吞噬活性,及孵育液中一氧化氮(NO)和免疫相关细胞因子表达量的影响。结果表明:LCS、LC、UB和EPS-2处理组较之对照组,均能显著增强头肾细胞的增殖能力和吞噬活性,HK处理组仅能显著增强头肾细胞的吞噬活性(P<0.05),其中LC(1.580±0.032)和LCS(1.520±0.058)分别对鲤头肾细胞增殖能力和吞噬活性具有最积极的促进作用。除CS组外,其余处理组均能显著提高孵育液中NO的合成量(P<0.05),EPS-2[(1.960±0.043)μmol/L]效果最为显著。所有处理组均能显著增加头肾细胞中细胞因子TNF-α、IL-1β、IL-6和TGF-β的合成量(P<0.05),并且分别是LCS[(23.11±1.31)pg/mL]、HK[(991.82±55.86)pg/mL]、LCS[(147.98±8.25)pg/mL]/CS[(147.38±7.94)pg/mL]和LCS[(139.56±9.69)pg/mL]处理组具有最佳的促进效果;而仅有LCS、CS和EPS-2处理组能显著提升IL-10的合成量(P<0.05),且LCS[(81.82±3.03)pg/mL]具有最佳的促进效果。研究表明,L.lactis Z-2的不同处理方式均对鲤表现出一定的免疫调节作用。To investigate the effects of the oral administration of different treatments for(mixture of live cells and supernatant,LCS;cell-free culture supernatant,CS;live cells,LC;heat-killed cells,HK,ultrasonic broken of cells,UB;exopolysaccharides of CS,EPS-2)L.lactis Z-2 on the probiotic performance in head kidney cells of Cyprinus carpio[(47.66±0.43)g],these different treatments of L.lactis Z-2 were co-cultured with the head kidney cells of the Cyprinus carpio.The proliferation and phagocytosis activition of nitric oxide(NO)and cytokines erpressionin the culture medium were determined.The re-sults showed that the preparation methed LCS,LC,UB and EPS-2 of L.lactis Z-2 significantlyenhanced the proliferation and phagocytic activity of the head kidney cells,HK treatment group only significantly enhanced the phagocytic activity of thehead kidney cells(P<0.05),LC(1.580±0.032)and LCS(1.520±0.058)had the most positive effect on theproliferation and phagocytic activity of head kidney cells,respectively.Except for CS group,the other treat-ment groups significantly increased the amount of NO synthesis in the incubation solution(P<0.05),the NOsynthesis effect of the EPS-2[(1.960±0.043)μmol/L]was the most significant.The synthesis of cytokines(TNF-α,IL-1β,IL-6 and TGF-β)in head kidney cells was significantly increased in all treatment groups(P<0.05),and the LCS[(23.11±1.31)pg/mL],HK[(991.82±55.86)pg/mL],LCS[(147.98±8.25)pg/mL]/CS[(147.38±7.94)pg/mL]and LCS[(139.56±9.69)pg/mL]treatment groups had the best promotion effect re-spectively.Only the LCS,CS and EPS-2 treatment groups could significantly increase the synthesis ofIL-10(P<0.05),and the LCS[(81.82±3.03)pg/mL]had the best promotion effect on IL-10.Researchshowd that,the different treatment methods of L.Lactis Z-2 all showed a certain immunomodulatory ef-fect on common carp,which provided a theoretical basis for the rational use of potential probiotics and anew solution for the application of lactic acid bacteria to microecological agents.

关 键 词：鲤 乳酸菌 头肾细胞 不同处理方式 免疫应答 

分 类 号：S816.32[农业科学—饲料科学]