Effects of sinew-regulating bone-setting manipulations on chondrocytes,IL-1βand NO in rabbits with knee osteoarthritis  

理筋正骨手法对膝骨关节炎模型兔软骨细胞及IL-1β和NO的影响

作　　者：Yao Meng-li Chen Zhao-hui Chen Xiang-hua Xu Han Wang Ting-ting Hu Rong-ting Jin Xiang-yu Jin Han 姚梦莉;陈朝晖;陈向华;徐寒;王婷婷;胡荣庭;金祥雨;金晗(Clinical College of Anhui Medical University,Hefei,230012,China;College of Acupuncture-moxibustion and Tuina,Anhui University of Chinese Medicine,Hefei,230038,China;Graduate School,Anhui University of Chinese Medicine,Hefei,230038,China)

机构地区：[1]Clinical College of Anhui Medical University,Hefei,230012,China [2]College of Acupuncture-moxibustion and Tuina,Anhui University of Chinese Medicine,Hefei,230038,China [3]Graduate School,Anhui University of Chinese Medicine,Hefei,230038,China

出　　处：《Journal of Acupuncture and Tuina Science》2021年第6期403-410,共8页针灸推拿医学（英文版）

基　　金：国家自然科学基金项目,No.81674076;安徽省教育厅自然基金重点项目,No.KJ2018A0273.

摘　　要：Objective To observe the therapeutic effect of sinew-regulating bone-setting manipulations for knee osteoarthritis(KOA)model rabbits and its impacts on the chondrocyte apoptosis rate and the levels of interleukin(IL)-1βand nitric oxide(NO).Methods According to the random number table method,30 New Zealand white rabbits were divided into a normal group(n=9)and a modeling group(n=21).Rabbits in the modeling group were used to establish KOA models with the modified Hulth method.At the 8th week,three rabbits were sacrificed to verify the model and the remaining 18 rabbits were randomly divided into a model group(n=9)and an intervention group(n=9).Rabbits in the normal group and model group were bred routinely without any intervention.Rabbits in the intervention group were treated with the sinew-regulating bone-setting manipulations,10 min/time,once every other day for a total of 20 times.The Lequesne MG knee function rating was used to evaluate the behavioral differences of the rabbits in each group.The Pelletier score was used to evaluate the general changes of the rabbits.The Mankin score was used to evaluate the pathology of knee cartilages.The enzyme-linked immunosorbent assay and nitrate reductase methods were used to determine the levels of IL-1βand NO in serum and synovial fluid of each group,respectively.In situ terminal deoxynucleotidyl transferase-mediated nick and labeling method was used to determine the apoptosis of chondrocytes in each group.Results Compared with the normal group,the scores of Lequesne MG,Pelletier and Mankin,and the levels of IL-1βand NO in the model group were increased(P<0.05),which indirectly indicated the success of the model.Compared with the model group,the scores of Lequesne MG,Pelletier and Mankin,IL-1βand NO levels,and chondrocyte apoptosis rate of the intervention group were decreased,and the differences were statistically significant(P<0.05).Conclusion The sinew-regulating bone-setting manipulations can reduce the levels of IL-1β,NO,and chondrocyte apoptosis rate,and de目的观察理筋正骨手法对膝骨关节炎(KOA)模型兔的治疗作用及对其软骨细胞凋亡率、白细胞介素(IL)-1β和一氧化氮(NO)浓度的影响。方法按照随机数字表法将30只新西兰大白兔进行分组,正常组9只,其余21只为造模组。造模组采用改良Hulth法建立KOA模型。8周时处死3只造模兔以验证模型是否成功。其余18只兔随机分为模型组和干预组,每组9只。正常组与模型组均常规饲养,不施加任何干预手段;干预组予以理筋正骨手法干预,每次10 min,隔日1次,共治疗20次。使用Lequesne MG膝关节功能评级评估各组兔的行为学差异,用Pelletier评分评估兔的大体变化,用Mankin评分评估膝关节软骨的病理情况,用酶联免疫吸附法和硝酸还原酶法分别测定各组血清和关节液中IL-1β和NO浓度,用原位末端标记法测定各组软骨细胞凋亡情况。结果与正常组相比,模型组的Lequesne MG、Pelletier和Mankin评分及IL-1β、NO浓度增加(P<0.05),间接提示造模成功。与模型组相比,干预组的Lequesne MG、Pelletier和Mankin评分,IL-1β和NO浓度,以及软骨细胞凋亡率下降,差异具有统计学意义(P<0.05)。结论理筋正骨手法可降低IL-1β及NO水平,减轻软骨细胞凋亡率,延缓关节软骨退行性变,对KOA具有良好的治疗作用。

关 键 词：TUINA MASSAGE Manual Therapies Nitric Oxide INTERLEUKIN-1 Apoptosis Osteoarthritis Knee Rabbit 

分 类 号：R2-03[医药卫生—中医学]