A Defective CXCL16/CXCR6 Axis Increases the Risk of Pregnancy Loss via the Abnormal Crosstalk between Decidual γδ T Cells and Trophoblasts  

作　　者：Deng-Xuan Fan Ming-Qing Li Wen-Jie Zhou Hong-Lan Huang Hui-Li Yang Cong-Jian Xu 

机构地区：[1]Department of Gynecology,Obstetrics and Gynecology Hospital,Fudan University,Shanghai 200011,China [2]Department of Obstetrics and Gynecology of Shanghai Medical School,Fudan University,Shanghai,200011,China [3]Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases,Fudan University,Shanghai,200011,China [4]NHC Key Lab of Reproduction Regulation(Shanghai Institute of Planned Parenthood Research),Hospital of Obstetrics and Gynecology,Fudan University,Shanghai,200080,China [5]Department of Obstetrics and Gynecology,Reproductive Medical Center,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200025,China

出　　处：《Reproductive and Developmental Medicine》2021年第3期129-139,共11页生殖与发育医学（英文版）

基　　金：This study was supported by the National Natural Science Foundation of China(NSFC)(No.81300552,92057119,31970798);the Innovation-oriented Science and Technology Grant from NPFPC Key Laboratory of Reproduction Regulation(CX2017-2);the Program for Zhuoxue of Fudan University(JIF157602);the Support Project for Original Personalized Research of Fudan University.

摘　　要：Objective::The maternal-fetal interface undergoes dynamic changes to allow the fetus to grow and develop in the uterus.The interaction between decidualγδT cells and trophoblasts plays a pivotal role during successful pregnancy;however,their physiological functions in early-term human pregnancy are still not completely illustrated.This study was undertaken to illustrate the functional roles of CXCL16/CXCR6 to prevent pregnancy loss via the crosstalk between decidualγδT cells and HTR8/SVneo trophoblast cells.Methods::The percentile of CXCR6+γδT cells in the peripheral blood from normal female and recurrent spontaneous abortion(RSA)patients was analyzed by flow cytometry.The expression of CXCR6 was detected in decidual immune cells via flow cytometry,and the expression of CXCL16 was analyzed in HTR8/SVneo trophoblast cells and lentivirus(LV)-HTR8/SVneo trophoblast cells via enzyme-linked immunosorbent assay.Reverse transcriptase-polymerase chain reaction was used to verify the expression of the CXCL16 gene in LV-HTR8/SVneo trophoblast cells.Expression of granzyme B and cytokines and proliferation of decidualγδT cocultured with HTR8/SVneo trophoblast cells were analyzed by flow cytometry.Invasion of HTR8/SVneo trophoblast cells was assessed via Matrigel transwell assay.Adoptive transfer was induced in vivo further to illustrate that the normal expression of CXCL16/CXCR6 could prevent pregnancy loss.Results::The percentile of CXCR6+γδT cells in the peripheral blood from RSA patients was lower than normal pregnancies.The expression of CXCR6 was highest in the decidualγδT cells among decidual immune cells,and the expression of CXCL16 increased as the amount of HTR8/SVneo trophoblast cells increased.Expression of granzyme B in the decidualγδT cells was downregulated by cocultured with HTR8/SVneo cells dependent of CXCL16,and HTR8/SVneo trophoblast cells induced the Th2 cytokines production in the decidualγδT cells.Both the expression of CXCR6 in the decidualγδT cells and proliferation of the decidualγ�

关 键 词：CXCL16 CXCR6 DecidualγδT Cells Maternal-fetal Interface Recurrent Spontaneous Abortion TROPHOBLASTS 

分 类 号：R714.2[医药卫生—妇产科学]