AK092375通过膀胱癌GLUT3的表达对NF-κB/TCS2/mTOR信号通路相关因子的影响  

Effects of AK092375 on NFκB/TCS2/mTOR signaling pathway related factors through the expression of GLUT3 in bladder cancer

作　　者：黄勇[1] 易发现[1] 任超 张宏[1] 宝诗琪 闫骏[1] Huang Yong;Yi Faxian;Ren Chao;Zhang Hong;Bao Shiqi;Yan Jun(Department of Urology, Affiliated Hospital of Inner Mongolia Medical University, Inner Mongolia, Hohhot 010000, China)

机构地区：[1]内蒙古医科大学附属医院泌尿外科,呼和浩特010000

出　　处：《疑难病杂志》2022年第2期192-197,共6页Chinese Journal of Difficult and Complicated Cases

基　　金：内蒙古自治区自然科学基金项目(2019MS08144)。

摘　　要：目的观察长链非编码RNA(lncRNA)AK092375在膀胱癌细胞中的表达及其通过葡萄糖转运蛋白3(GLUT3)的表达对NF-κB/TCS2/mTOR信号通路相关因子的影响。方法2020年1月—2021年5月于内蒙古医科大学附属医院临床医学研究中心进行相关实验。选择AK092375表达量最低的膀胱癌细胞系T24作为研究对象,并与人正常膀胱上皮细胞SV-H、UC-1进行比较。取培养生长良好的T24细胞随机分为A组(AK092375和GLUT3同时过表达)、B组(AK092375和GLUT3同时干扰)、C组(AK092375过表达而GLUT3干扰)、D组(AK092375干扰而GLUT3过表达)4组。通过RT-PCR和Western-blot法检测各组细胞GLUT3及下游NF-κB/TCS2/mTOR信号通路(NF-κB、TCS2、mTOR)mRNA和蛋白表达水平;并分析各组膀胱癌细胞的增殖和迁移情况。结果Western-blot检测结果显示,AK092375和GLUT3蛋白在人膀胱癌细胞系T24的表达量明显高于人正常膀胱上皮细胞SV-HUC-1(t/P=3.215/<0.001、2.538/<0.001)。GLUT3、NF-κB、TCS2、mTOR mRNA和蛋白表达水平比较,A组>D组>C组>B组,且各组间比较差异有统计学意义(mRNA:F/P=25.940/<0.001、28.444/<0.001、23.919/<0.001、16.853/<0.001;蛋白:F/P=7.070/<0.001、7.497/<0.001、33.717/<0.001、12.519/<0.001)。膀胱癌细胞的迁移率和增殖情况比较,A组>D组>C组>B组,各组间比较差异均有统计学意义(F/P=9.704/<0.001,3.265/<0.001)。结论AK092375可能通过上调GLUT3的表达,从而激活下游NFκB/TCS2/mTOR信号通路,进而促进膀胱癌细胞的增殖和转移。Objective To observe the expression of long non-coding RNA(lncRNA)AK092375 in bladder cancer cells and its effect on NFκB/TCS2/mTOR signaling pathway related factors through the expression of glucose transporter 3(GLUT3).Methods From January 2020 to May 2021,relevant experiments were conducted at the Clinical Medical Research Center of the Affiliated Hospital of Inner Mongolia Medical University.The bladder cancer cell line T24 with the lowest expression of AK092375 was selected as the research object,and compared with human normal bladder epithelial cells SV H and UC 1.The well-grown T24 cells were randomly divided into group A(overexpression of AK092375 and GLUT3),group B(interference of AK092375 and GLUT3 at the same time),group C(overexpression of AK092375 and interference of GLUT3),group D(interference of AK092375 and overexpression of GLUT3)expression 4 groups.RT PCR and Western blot were used to detect the mRNA and protein expression levels of GLUT3 and downstream NFκB/TCS2/mTOR signaling pathway(NFκB,TCS2,mTOR)in each group of cells;and the proliferation and migration of bladder cancer cells in each group were analyzed.Results Western blot results showed that the expression levels of AK092375 and GLUT3 proteins in human bladder cancer cell line T24 were significantly higher than those in human normal bladder epithelial cell SV HUC1(t/P=3.215/<0.001,2.538/<0.001).The mRNA and protein expression levels of GLUT3,NF-κB,TCS2,mTOR were compared,group A>group D>group C>group B,and there were significant differences among the groups(mRNA:F/P=25.940/<0.001,28.444/<0.001,23.919/<0.001,16.853/<0.001;protein:F/P=7.070/<0.001,7.497/<0.001,33.717/<0.001,12.519/<0.001).In comparison of the migration rate and proliferation of bladder cancer cells,group A>group D>group C>group B,and there were significant differences among the groups(F/P=9.704/<0.001,3.265/<0.001).Conclusion AK092375 may promote the proliferation and metastasis of bladder cancer cells by up-regulating the expression of GLUT3,thereby activating the dow

关 键 词：膀胱癌 长链非编码RNA AK092375 葡萄糖转运蛋白3 NF-κB/TCS2/mTOR信号通路 

分 类 号：R737.14[医药卫生—肿瘤]