全局调控因子LaeA对蛹虫草生长发育和次生代谢的作用  

Effects of LaeA on Growth,Development and Secondary Metabolism of Cordyceps militaris

作　　者：汪滢[1] 杨占山 鲍大鹏[1] 李燕[1] 吴莹莹[1] 邹根 WANG Ying;YANG Zhanshan;BAO Dapeng;LI Yan;WU Yingying;ZOU Gen(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,National Engineering Research Center of Edible Fungi,Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China,Shanghai 201403,China)

机构地区：[1]上海市农业科学院食用菌研究所,国家食用菌工程技术研究中心,农业农村部南方食用菌资源利用重点实验室,上海201403

出　　处：《食用菌学报》2022年第1期20-26,共7页Acta Edulis Fungi

基　　金：上海市科技兴农重点攻关项目(2018-02-08-00-12-F01555);国家自然科学基金(31902089)。

摘　　要：通过克隆蛹虫草(Cordyceps militaris)野生型菌株Cm01的laeA基因(CmLaeA)、合成构巢曲霉(Aspergillus nidulans)的laeA基因(AsLaeA),构建不同过表达载体,利用农杆菌(Agrobacterium tumefaciens)介导转化野生型蛹虫草菌株LN9、Cm01,获得4个过表达菌株,分别命名为CmLaeALN9(CmLaeA基因转化菌株LN9)、AsLaeALN9(AsLaeA基因转化菌株LN9)、CmLaeACm01(CmLaeA基因转化菌株Cm01)、AsLaeACm01(AsLaeA基因转化菌株Cm01),结果表明:与野生型菌株相比,过表达菌株CmLaeACm01、AsLaeALN9、AsLaeACm01的虫草素含量提高(2.5倍),CmLaeACm01的分生孢子产量提高(1.5倍)、出草时间提前5~7 d。LaeA的调控作用因不同来源的LaeA和不同的宿主菌而异。The laeA genes of the wild type Cordyceps militaris Cm01(CmLaeA)and Aspergillus nidulans(AsLaeA)were cloned and synthesized,respectively,and then used to construct corresponding overexpression vectors.Using Agrobacterium tumefaciens mediated transformation,these overexpression vectors were then transferred into wild C.militaris strains Cm01 and LN9 respectively to generate four recombinant strains as follows:CmLaeALN9(transformation of LN9 with CmLaeA),CmLaeACm01(transformation of Cm01 with CmLaeA),AsLaeALN9(transformation of LN9 with AsLaeA)and AsLaeACm01(transformation of Cm01 with AsLaeA).Compared with the wild type strains,CmLaeACm01,AsLaeALN9 and AsLaeACm01 contained 1.5 times more cordycepin.CmLaeAcm01 produced 50%more conidia and started to develop fruiting bodies 5-7 d earlier than the wild type strains.The regulatory effect of LaeA varies with source and host strain.

关 键 词：蛹虫草 LAEA 子实体发育 虫草素 

分 类 号：S567.3[农业科学—中草药栽培]