一个云芝菌株的分离及栽培条件优化  

Isolation and Domestication of a Trametes versicolor Strain

作　　者：夏志兰[1,2,3] 马鑫旺 熊昱静 刘飞 谢玲 XIA Zhilan;MA Xinwang;XIONG Yujing;LIU Fei;XIE Ling(Horticulture College,Hunan Agricultural University,Changsha 410128,Hunan,China;Key Laboratory for Vegetable Biology of Hunan Province,Changsha 410128,Hunan,China;Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding,Ministry of Education,Changsha 410128,Hunan,China;Guangdong Jizhi Yunzhi Pharmaceutical Technology Co.,Ltd.,Guangzhou 510385,Guangdong,China)

机构地区：[1]湖南农业大学园艺学院,湖南长沙410128 [2]蔬菜生物学湖南省重点实验室,湖南长沙410128 [3]园艺作物种质创新与新品种选育教育部工程研究中心,湖南长沙410128 [4]广东集智云芝医药科技有限公司,广东广州510385

出　　处：《食用菌学报》2022年第1期48-54,共7页Acta Edulis Fungi

基　　金：湖南省科技厅自然科学基金(2020JJ4366)。

摘　　要：对采自湖南省长沙橘子洲头柳树腐木上的野生云芝(Trametes versicolor)进行组织分离和鉴定,并优化分离菌株的栽培条件。利用60%杜仲木屑、20%棉籽壳、17%麦麸、1%蔗糖、1%碳酸钙、1%石膏栽培云芝分离菌株,在螺旋状开袋方式下,生物学效率达84.62%,获得的云芝中多糖含量5.9%,水溶性浸出物含量23.9%,麦角甾醇含量3.1‰,多糖含量和水溶性浸出物含量分别是《中华人民共和国药典》中云芝质量标准的1.84倍和1.33倍。A wild strain of Trametes versicolor collected from rotten willow trees in Juzizhoutou, Changsha, Hunan province was isolated, identified and then domesticated. The results showed that the strain was identified as T. versicolor based on its morphological characteristics and 100% similarity between its ITS sequence(GenBank accession number: OL831142) and the published ITS sequences of T. versicolor in NCBI. The substrate for domesticated cultivation comprised 60% Eucommia ulmoides sawdust, 20% cottonseed hull, 17% wheat bran, 1% sucrose, 1% calcium carbonate and 1% gypsum. Using the above mentioned substrate and spiral bag opening method, the biological efficiency of T. versicolor reached 84.62%, and the resultant fruiting bodies contained 5.9% polysaccharides, 23.9% water-soluble extract and 3.1‰ ergosterol. The polysaccharide content and water-soluble extract content were 1.84 and 1.33 times the quality standards of the Chinese Pharmacopoeia(2020 edition), respectively.

关 键 词：云芝 ITS鉴定 栽培 云芝多糖 水溶性浸出物 麦角甾醇 

分 类 号：S567.3[农业科学—中草药栽培]