芬太尼对结直肠癌小鼠瘤体大小及血管内皮生长因子-A、缺氧诱导因子-1α的影响  

Effects of Fentanyl on Tumor Volume,Vascular Endothelial Growth Factor-A and Hypoxia Inducible Factor-1αin Mice with Colorectal Cancer

作　　者：吴昱 张占峰 郝书航 汪国基 底妍 汤龙信 WU Yu;ZHANG Zhan-feng;HAO Shu-hang;WANG Guo-ji;DI Yan;TANG Long-xin(Department of Anesthesiology,the 980th Hospital of PLA Joint Logistics Support Forces,Shijiazhuang 050082,China)

机构地区：[1]解放军联勤保障部队第九八〇医院麻醉科,石家庄050082

出　　处：《临床误诊误治》2022年第2期102-106,共5页Clinical Misdiagnosis & Mistherapy

基　　金：河北省卫计委医学重点项目(ZD20170953);解放军白求恩国际和平医院青年课题项目(2016-30)。

摘　　要：目的探讨芬太尼对结直肠癌小鼠肿瘤大小及血管内皮生长因子-A(VEGF-A)、缺氧诱导因子-1α(HIF-1α)的影响。方法选择15只6~8周龄雄性BALB/C小鼠,采用右前肢腋窝皮下注射结肠癌细胞株CT26的方法制作结直肠癌小鼠模型,肿瘤直径>1 cm时,按照随机数字表法分为A、B、C组,每组5只。B组和C组分别给予芬太尼10和50μg/(kg·d)腹腔注射,A组给予等量生理盐水腹腔注射,连续1周。颈椎脱臼法处死小鼠,观察3组肿瘤直径和体积,比较血清VEGF-A和HIF-1α水平,以及瘤体组织VEGF-A、HIF-1αmRNA和蛋白表达情况。结果B组和C组肿瘤直径和体积均小于A组,血清VEGF-A和HIF-1α水平均低于A组,瘤体组织VEGF-A、HIF-1αmRNA和蛋白表达水平均低于A组(P<0.05)。C组瘤体组织VEGF-A蛋白表达水平低于B组(P<0.05);但B组与C组其余指标比较差异均无统计学意义(P>0.05)。结论芬太尼能够抑制结直肠癌小鼠肿瘤的生长,这种抑制作用可能是通过下调VEGF-A和HIF-1α实现的,而且该作用可能与芬太尼的剂量相关。Objective To investigate effects of Fentanyl on tumor volume,vascular endothelial growth factor-A(VEGF-A)and hypoxia-inducible factor-1α(HIF-1α)in mice with colorectal cancer(CRC).Methods In this study,15 male BALB/C mice aged 6-8 weeks were selected,and CRC mouse models were constructed by subcutaneous injection of colon cancer cell line CT26 into the axilla of the right forelimb.When the tumor diameter was larger than 1 cm,the mice were divided into group A,B and C(n=5 in each group)according to the random number table.Group B and C were intraperitoneally injected with Fentanyl 10 and 50μg/(kg·d)respectively,while group A was intraperitoneally injected with the same volume of normal saline,all the groups were treated for 1 week.The mice were sacrificed by cervical dislocation.Tumor diameters and volumes were observed,and serum levels of VEGF-A and HIF-1α,as well as mRNA and protein expressions of VEGF-A and HIF-1αin tumor tissues were compared among three groups.Results In group B and C,tumor diameters and volumes were significantly smaller,and serum levels of VEGF-A and HIF-1αwere significantly lower,and mRNA and protein expressions of VEGF-A and HIF-1αin tumor tissues were significantly lower than those in group A(P<0.05).The expression of VEGF-A protein in tumor tissue in group C was significantly lower than that in group B(P<0.05);however,there were no other significant differences in other indicators between group B and C(P>0.05).Conclusion Fentanyl may inhibit tumor growth in mice with CRC,which may be realized by down-regulation of VEGF-A and HIF-1α,and the effect may be related to the dose of Fentanyl.

关 键 词：结直肠肿瘤 芬太尼 血管内皮生长因子-A 缺氧诱导因子-1Α 小鼠 近交BALB/C 

分 类 号：R735.34[医药卫生—肿瘤]