高危型HPV持续感染者宫颈局部调节性T淋巴细胞表达  

Analysis of the expression of local regulatory T lymphocytes in the cervix of patients with persistent human papilloma virus infection

作　　者：林一禾 朱淑嫔 陈游沓 LIN Yihe;ZHU Shubin;CHEN Youda(Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine, Zhejiang Province, 325000)

机构地区：[1]浙江省温州市中西医结合医院,325000

出　　处：《中国计划生育学杂志》2021年第10期2208-2210,2214,共4页Chinese Journal of Family Planning

摘　　要：目的:分析人乳头瘤病毒(HPV)持续感染与宫颈局部调节性T淋巴细胞(Treg)关系。方法:选择2018年5月—2020年10月本院收治的高危型HPV阳性患者476例,根据随访结果分为HPV持续感染组(178例)与HPV转阴组(298例),应用流式细胞技术检测Treg,采用杂交捕获2代(HC-Ⅱ)方法检测HPV-DNA。结果:持续感染组Treg(8.2±2.0)%高于转阴组(6.1±3.8)%(P<0.05);持续感染组年龄≥35岁、多重HPV感染、宫颈活检为低度鳞状上皮内瘤变(LSIL)者Treg较高(P<0.05),体质指数不同者间未见差异(P>0.05)。年龄≥35岁、多重HPV感染、Treg≥7.8%为HPV持续感染的独立高危因素(P<0.05);Treg对HPV持续感染具有预测效能(P<0.001),AUC为0.834(95%CI:0.754-0.914),特异性为69.1%,灵敏度为73.4%。结论:HPV持续感染与宫颈免疫微环境有关,宫颈局部Treg对HPV的持续感染有预测价值。Objective:To analyze the relationship between persistent human papillomavirus(HPV)infection of the patients and their expression of local regulatory T lymphocytes(Treg)in the cervix.Methods:A total of 476 patients with HPV positive were selected as the research subjects from May 2018 to October 2020.According to the results of followed up,these patients were divided into group A(178 patients with persistent HPV infection)and group B(298 patients with HPV turning to negative).The expression of Treg was detected by flow cytometry,and HPV-DNA was detected by hybridization capture 2 generation(HC-Ⅱ).Results:The expression of Treg(8.2±2.0%)of the patients in group A was significant higher than that(6.1±3.8%)of the patients in group B(P<0.05).In group A,the expression of Treg of the patients with≥35 years old,the patients with multiple HPV infection,or the patients with low grade squamous intraepithelial neoplasia(LSIL)by cervical biopsy was significant higher(P<0.05),but which had no significant difference between the patients with difference(≥24kg/m2 or<24kg/m2)of body mass index(P>0.05).Age≥35 years old,multiple HPV infection,and Treg≥7.8%of the patients were the independent risk factors of their persistent HPV infection(P<0.05).The expression of Treg for persistent HPV infection had predictive efficacy(P<0.001),and the AUC,the specificity,and the sensitivity of which were 0.834(95%CI:0.754-0.914),69.1%,and 73.4%,respectively.Conclusion:The persistent infection of HPV of the patients is related to the immune microenvironment of their cervix,and local Treg expression in the cervix is valuable for predicting the persistent infection of HPV.

关 键 词：宫颈疾病 高危型人乳头瘤病毒 持续感染 调节性T淋巴细胞 

分 类 号：R737.33[医药卫生—肿瘤]