假喜马拉雅块菌化学成分分析及其抗氧化能力研究  

The analysis of the antioxidant activities and chemical constituents of Tuber pesudohimalayense

作　　者：李新爱 郭文秀 张烨 张旭[1] 尹胜 王欣宇 郭大乐 邓赟[1] LI Xinai;GUO Wenxiu;ZHANG Ye;ZHANG Xu;YIN Sheng;WANG Xinyu;GUO Dale;DENG Yun(School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Huidong Takachuan Tianyuan Agricultural Technology Co.Ltd.,Liangshan Yi Autonomous Prefecture 615000,China)

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]会东高川天源农业科技有限公司,四川凉山彝族自治州615000

出　　处：《食品与发酵工业》2022年第4期185-192,共8页Food and Fermentation Industries

基　　金：四川省科技厅应用基础研究项目(2018JY0186)。

摘　　要：通过超高效液相色谱-四极杆-静电场轨道阱高分辨质谱法(ultra-performance liquid chromatography-tandem q-exactive quadrupole-electrostatic field track trap high resolution mass spectrometry,UPLC-Q-Orbitrap HRMS)化学成分分析和体外抗氧化活性(DPPH自由基、ABTS阳离子自由基、羟自由基清除率,还原能力及总酚含量)测定,对假喜马拉雅块菌子实体80%(体积分数)甲醇提取物的主要营养成分和子实体不同溶剂(80%甲醇、水)提取物的体外抗氧化能力进行初步研究。从假喜马拉雅块菌中共鉴定出51个成分,包括13个游离氨基酸、11个有机酸、8个核苷类、9个酰胺类、10个其他类,其中8个成分(苯丙氨酸、亮氨酸、L-谷氨酸、L-酪氨酸、腺苷、苯甲酸、油酰胺、吡嗪)经与对照品比对后准确鉴别。抗氧化结果表明,水提物对DPPH自由基、ABTS阳离子自由基、羟自由基清除率及还原力较高,EC_(50)值分别为14.97、0.19、3.21 mg/mL、水提物的总酚含量最高3.99 mg/g,甲醇提取物的总酚含量1.87 mg/g。该研究表明块菌不同的提取物中总酚含量及其抗氧化活性存在一定差异性且总酚含量与抗氧化活性呈现出一定的正相关性。该研究可为块菌质量评价、化学成分及其药理活性基础研究奠定基础,为天然抗氧化剂的开发提供科学依据,对开发利用块菌资源具有指导意义。Through ultra-performance liquid chromatography-tandem q-exactive quadrupole-electrostatic field track trap high resolution mass spectrometry(UPLC-Q-Orbitrap HRMS HRMS)analysis and in vitro antioxidant activity(DPPH radicals,ABTS cationic radicals,hydroxyl scavenging rate,reducing power and total phenolic content)determination,the main nutrients of the 80%methanol extract of the Tuber pesudohimalayense and the antioxidant capacity of different solvents of the fruiting body(80%methanol,water)extract were preliminarily studied.A total of 51 components were identified from T.pesudohimalayense,including 13 free amino acids,11 organic acids,8 nucleosides,9 amides,and 10 other types,8 components(phenylalanine,leucine,L-glutamic acid,L-tyrosine,adenosine,benzoic acid,oleamide,pyrazine)were unambiguously identified by comparing with reference standards.The antioxidant results showed that the water extract(H_(2)O)had higher scavenging and reducing power activities against DPPH free radicals,ABTS free radicals,and hydroxyl free radicals.The EC_(50) values were 14.97,0.19,3.21 mg/mL,respectively.The total phenol content of the water extract(H_(2)O)was the highest(3.99 mg/g),and the total phenol content of the methanol extract(ME)was 1.87 mg/g.The study showed that the total phenol content and antioxidant activity in different extracts of truffles were different,and the total phenol content and antioxidant activity showed a positive correlation.This research could lay the foundation for the quality evaluation of truffles,the basic research of chemical composition and pharmacology,which providing a scientific basis for the development of natural antioxidants,and guiding significance for the development and utilization of truffle resources.

关 键 词：假喜马拉雅块菌 超高效液相色谱-四极杆-静电场轨道阱高分辨质谱法 氨基酸 总酚 抗氧化活性 

分 类 号：O657.63[理学—分析化学] TS219[理学—化学]