基于^(1)H-NMR及GC-MS的中国块菌及其共生云南松的代谢物分析  

Analyses of metabolites of Tuber sinense and its symbiotic Pinus yunnanensis based on ^(1)H-NMR and GC-MS

作　　者：周雪 袁鹏宇 安霜 陈鹏至 陈幽幽 杨晓敏 李宗菊[1] ZHOU Xue;YUAN Pengyu;AN Shuang;CHEN Pengzhi;CHEN Youyou;YANG Xiaomin;LI Zongju(School of Ecology and Environmental Science of Yunnan University,Kunming 650500,Yunnan,China;School of Life Sciences of Yunnan University,Kunming 650500,Yunnan,China)

机构地区：[1]云南大学生态与环境学院,云南昆明650500 [2]云南大学生命科学学院,云南昆明650500

出　　处：《菌物学报》2022年第1期105-123,共19页Mycosystema

基　　金：国家自然科学基金(31560575)。

摘　　要：采用代谢组学技术研究中国块菌(云南黑松露)及其共生云南松的小分子活性代谢产物。运用核磁共振(^(1)H-NMR)和气相质谱(GC-MS)两种技术,分析新鲜中国块菌成熟子实体及松根的代谢成分;将^(1)H-NMR及GC-MS数据导入SMICA-P软件进行多元统计分析,比较二者的差异代谢物。在块菌子实体及松根中分别共检测到了苏氨酸、甘露二糖、延胡索酸、茶碱和表儿茶素等64个及45个化合物,其中松醇、1-环己烯-1-甲酸、谷氨酸、海藻糖、甜菜碱等25种物质具有显著差异,这些差异物质以碳代谢及氮代谢为主。两种方法互为补充,扩大了检测范围。本研究为探明中国块菌与其共生松树的共生机理,为今后中国块菌相关药物的开发及人工培养的研究提供了理论依据。Small molecule active metabolites of Tuber sinense(Yunnan black truffle)and its symbiotic Pinus yunnanensis were studied by metabonomics technique.Nuclear magnetic resonance(^(1)H-NMR)and gas phase mass spectrometry(GC-MS)were used to analyze the metabolic components of mature fruit-body of fresh Tuber sinense and pine root.The ^(1)H-NMR and GC-MS data were imported into SMICA-P software for multivariate statistical analysis,and the differential metabolites between them were compared.A total of 64 and 45 compounds,including threonine,mannobiose,fumaric acid,theophylline and epicatechin,was detected in truffle and pine roots,respectively,among which,25 compounds,such as terpenol,1-cyclohexene-1-formic acid,glutamate,trehalose and betaine,showed significant differences,and these differential compounds were mainly related to carbon metabolism and nitrogen metabolism.Mutual complement of the two methods can expand the detection range.This study provides a reference for exploring the symbiotic mechanism of Tuber sinense and its symbiotic pine and developing Tuber sinense related drugs and artificial culture in future.

关 键 词：中国块菌 云南松 松根 核磁共振 气相色谱质谱联用 代谢物 

分 类 号：S567.3[农业科学—中草药栽培]