Enhanced Biodegradation of High-Salinity and Low- Temperature Crude-Oil Wastewater by Immobilized Crude-Oil Biodegrading Microbiota  

作　　者：HUANG Xiao ZHOU Ting CHEN Xi BAI Jie ZHAO Yangguo 

机构地区：[1]Collaborative Innovation Center of Atmospheric Environment and Equipment Technology,Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control,School of Environmental Science and Engineering,Nanjing University of Information Science and Technology,Nanjing 210044,China [2]The Key Laboratory of Marine Environmental Science and Ecology,Ministry of Education,Ocean University of China,Qingdao 266100,China [3]Harbin Institute of Technology(Shenzhen),Shenzhen Key Laboratory of Water Resource Utilization and Environmental Pollution Control,Shenzhen 518055,China

出　　处：《Journal of Ocean University of China》2022年第1期141-151,共11页中国海洋大学学报（英文版）

基　　金：supported by the National Key R&D Program of China(No.2018YFD0900805);the Start up Foundation for Introducing Talent of Nanjing Univer-sity of Information Science and Technology。

摘　　要：High salt and low temperature are the bottlenecks for the remove of oil contaminants by enriched crude-oil degrading microbiota in Liaohe Estuarine Wetland(LEW),China.To improve the performance of crude-oil removal,microbiota was further immobilized by two methods,i.e.,sodium alginate(SA),and polyvinyl alcohol and sodium alginate(PVA+SA).Results showed that the crude oil was effectively removed by the enrichment with an average degrading ratio of 19.42-31.45 mg(L d)^(−1).The optimal inoculum size for the n-alkanes removal was 10%and 99.89%.Some members of genera Acinetobacter,Actinophytocola,Aquabac-terium,Dysgonomonas,Frigidibacter,Sphingobium,Serpens,and Pseudomonas dominated in crude-oil degrading microflora.Though the removal efficiency was lower than free bacteria when the temperature was 15℃,SA and PVA+SA immobilization im-proved the resistance to salinity.The composite crude-oil degrading microbiota in this study demonstrated a perspective potential for crude oil removal from surface water under high salinity and low temperature conditions.

关 键 词：crude-oil degrading microbiota microbial community IMMOBILIZATION high salinity low temperature 

分 类 号：X172[环境科学与工程—环境科学] X74