流式细胞术在多发性骨髓瘤治疗中的应用价值  

Application Value of Flow Cytometry in the Treatment of Multiple Myeloma

作　　者：邓娜[1] 吴冠宇[1] 杨柳[1] 谷和先[1] 盛德菁 周娜 王何惧 DENG Na;WU Guan-yu;YANG Liu;GU He-xian;SHENG De-jing;ZHOU Na;WANG He-ju(Department of Hematology,Maanshan People's Hospital,Maanshan 243000,Anhui,China)

机构地区：[1]马鞍山市人民医院血液科,安徽马鞍山243000

出　　处：《医学信息》2022年第2期75-78,共4页Journal of Medical Information

基　　金：马鞍山市科技局项目(编号:KY2017-012)。

摘　　要：目的使用流式细胞仪分析免疫表型在初诊多发性骨髓瘤中的特点,并分析在疾病不同分型及分期中表达类型的差异及其与预后的关系。方法选取2015年4月-2020年3月我院收治的34例初诊多发性骨髓瘤患者为研究对象,使用双激光四色流式细胞仪分析免疫表型,比较不同分期、不同分型的多发性骨髓瘤疾病中的表达差异。结果CD38、CD138阳性率为100.00%。CD45、CD38、CD19、CD56、CD20、CD28、CD117、CD9、CD23的表达与疾病分型无关(P>0.05),CD33的表达与疾病分型有关(P<0.05)。CD45、CD38、CD56、CD20、CD28、CD117、CD9、CD23、CD33的表达与ISS分期无关(P>0.05),CD19的表达与ISS分期有关(P<0.05)。结论免疫表型的分析更符合诊断标准,能更准确的区分良、恶性浆细胞,对于多发性骨髓瘤的预后及治疗后的监测均有临床意义。Objective To analyze the characteristics of immunophenotype in newly diagnosed multiple myeloma by flow cytometry,and to analyze the difference of expression types in different types and stages of the disease and its relationship with prognosis.Methods A total of 34 patients with newly diagnosed multiple myeloma in our hospital from April 2015 to March 2020 were selected as the research objects.The immunophenotype was analyzed by double laser four-color flow cytometry,and the expression differences of multiple myeloma diseases in different stages and different types were compared.Results The positive rates of CD38 and CD138 were 100.00%.The expression of CD45,CD38,CD19,CD56,CD20,CD28,CD117,CD9 and CD23 was not correlated with disease classification(P>0.05);the expression of CD33 was correlated with disease classification(P<0.05).The expression of CD45,CD38,CD56,CD20,CD28,CD117,CD9,CD23 and CD33 was not correlated with ISS stage(P>0.05);the expression of CD19 was correlated with ISS stage(P<0.05).Conclusion The analysis of immunophenotype is more consistent with the diagnostic criteria and can distinguish benign and malignant plasma cells more accurately,which is of significance for the prognosis and post-treatment monitoring of multiple myeloma.

关 键 词：流式细胞术 免疫表型 多发性骨髓瘤 

分 类 号：R738.1[医药卫生—肿瘤]