环磷酰胺联合环孢霉素介导的获得性再生障碍性贫血小鼠模型的建立  

Establishment of a Mouse Model of Acquired Aplastic Anemia Mediated by Cyclophosphamide Combined with Cyclosporine

作　　者：石健菲 焦丹丽 赵琛[1] 戚莉 李蔚江[1] SHIJian-Fei;JIAO Dan-Li;ZHAO Chen;QI Li;LI Wei-Jiang(College of Acupuncture-Moxibustion and Tuitia,Shanghai University of Traditonal Chinese Medicine,Shanghai 201203,China;Institute of Interdisciplinary Integrative Medicine Research,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学针灸推拿学院,上海201203 [2]上海中医药大学交叉科学研究院,上海201203

出　　处：《中国实验血液学杂志》2022年第1期222-226,共5页Journal of Experimental Hematology

基　　金：上海中医药大学医养结合科创项目。

摘　　要：目的:建立较稳定的获得性再生障碍性贫血小鼠模型。方法:雌性6月龄BALB/C小鼠经连续14 d腹腔注射环磷酰胺和环孢霉素后,观察其外周血三系细胞数、血红蛋白浓度以及骨髓有核细胞数量、骨髓涂片、骨髓病理切片等指标。结果:经腹腔注射环磷酰胺和环孢霉素的BALB/C小鼠外周血三系细胞数及血红蛋白浓度均显著减少,其中白细胞、血小板表现得尤为明显;骨髓涂片示骨髓有核细胞数量明显下降,骨髓增生低下;骨髓病理切片示造血细胞减少,脂肪细胞等非造血细胞增多。结论:通过环磷酰胺联合环孢霉素腹腔注射的小鼠模型,其各项检测指标均达到获得性再生障碍性贫血的诊断标准,可作为获得性再生障碍性贫血发病机制及治疗研究的小鼠模型。Objective:To establish a stable mouse model of acquired aplastic anemia.Methods:Female BALB/C mice aged 6 months were intraperitoneally injected with cyclophosphamide and cyclosporine for 14 days.The number of peripheral blood cells,the concentration of hemoglobin,the number of bone marrow nucleated cells,bone marrow smear,bone marrow pathological sections and other indexes were observed.Results:In BALB/C mice injected intraperitoneally with cyclophosphamide and cyclosporine,the number of peripheral blood cells and the concentration of hemoglobin were significantly decreased,especially the white blood cells and platelets.Bone marrow smear showed a significant decrease in the number of nucleated cells and bone marrow hyperplasia.Bone marrow pathology showed decreased hematopoietic cells and increased non-hematopoietic cells such as adipocytes.Conclusion:The mouse model with intraperitoneal injection of cyclophosphamide and cyclosporine can meet the diagnostic criteria of acquired aplastic anemia,which can be used as a mouse model for the study of the pathogenesis and treatment of acquired aplastic anemia.

关 键 词：获得性再生障碍性贫血 环磷酰胺 环孢霉素 小鼠模型 

分 类 号：R733.71[医药卫生—肿瘤]