肝内胆管癌组织胆汁酸G蛋白偶联受体5和热休克蛋白75表达及与预后的关系  

Expressions of takeda G protein-coupled receptor 5 and mortalin protein 75 in intrahepatic cholangiocarcinoma and their relationship with prognosis

作　　者：赵帅华[1] 张先舟 蒙博 曹胜利[3] ZHAO Shuaihua;ZHANG Xianzhou;MENG Bo;CAO Shengli(Department of Pathology,Anyang District Hospital,Puyang City,Anyang,Henan 455000,P.R.China;Department of Hepatobiliary and Pancreatic Surgery,Tumor Hospital Affiliated to Zhengzhou University,Zhengzhou 450008,P.R.China;Department of Hepatobiliary and Pancreatic Surgery,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,P.R.China)

机构地区：[1]濮阳市安阳地区医院病理科,河南安阳455000 [2]郑州大学附属肿瘤医院肝胆胰腺外科,郑州450008 [3]郑州大学第一附属医院肝胆胰外科,郑州450052

出　　处：《中国普外基础与临床杂志》2022年第2期218-225,共8页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的检测肝内胆管癌(intrahepatic cholangiocarcinoma,ICC)患者癌组织中胆汁酸G蛋白偶联受体5(takeda G protein-coupled receptor 5,TGR5)和热休克蛋白75的表达情况,探讨二者与患者预后的关系。方法选取2015年3月至2018年3月期间濮阳市安阳地区医院收治住院并行手术治疗的ICC患者94例作为研究对象,采用免疫组织化学法及Western blot(WB)法检测ICC癌组织及其癌旁组织中TGR5蛋白和热休克蛋白75的表达情况,分析ICC癌组织中TGR5蛋白及热休克蛋白75表达情况与ICC临床病理特征及预后的关系;并采用多因素Cox比例风险回归分析ICC患者预后不良的危险因素,采用ROC曲线分析TGR5蛋白和热休克蛋白75表达水平对ICC患者预后不良的诊断价值。结果免疫组织化学结果显示,ICC癌组织中TGR5蛋白和热休克蛋白75表达阳性率均高于癌旁组织(P<0.05)。WB结果显示,ICC癌组织中TGR5蛋白和热休克蛋白75相对表达量高于癌旁组织(P<0.05)。ICC患者癌组织中TGR5蛋白的表达与患者性别、肿瘤直径、分化程度、TNM分期、卫星灶和肝硬变相关(P<0.05);热休克蛋白75的表达与肿瘤直径、TNM分期、神经受累、卫星灶和肝硬变相关(P<0.05)。预后不良组和预后良好组患者在性别、肿瘤直径、TNM分期、微血管侵犯、卫星灶、肝硬变以及TGR5蛋白和热休克蛋白75表达情况方面比较,差异有统计学意义(P<0.05)。TGR5蛋白表达阳性患者的累积3年总生存率(32.00%)低于TGR5蛋白表达阴性患者(63.16%),χ^(2)=6.228,P=0.013;热休克蛋白75表达阳性患者的累积3年总生存率(32.91%)低于热休克蛋白75表达阴性患者(66.67%),χ^(2)=6.079,P=0.014。多因素Cox比例风险回归分析结果显示,TGR5蛋白和热休克蛋白75表达阳性、TNMⅢ+Ⅳ期以及有卫星灶和肝硬变为ICC患者预后不良的危险因素(P<0.05)。ROC结果显示,以TGR5蛋白表达水平0.932为截断值,诊断ICC患者预后不良的AUC为0.783,敏感度为Objective To detect the expressions of takeda G protein-coupled receptor 5(TGR5)and mortalin protein 75 in the tissues of patients with intrahepatic cholangiocarcinoma(ICC),and to explore their relationship with prognosis.Methods A total of 94 ICC patients who were admitted to Anyang District Hospital and received surgical treatment from March 2015 to March 2018 were selected as the research objects.The expressions of TGR5 and mortalin protein 75 in ICC cancer tissues and adjacent tissues were detected by immunohistochemistry and Western blot(WB).The relationship between the expressions of TGR5 and mortalin protein 75 in ICC cancer tissues and clinicopathological parameters and prognosis was analyzed.Multivariate Cox proportional hazards regression was used to analyze the risk factors of poor prognosis in patients with ICC.ROC curve was used to analyze the diagnostic value of TGR5 and mortalin protein 75 for poor prognosis in patients with ICC.Results Immunohistochemical results showed that the positive expression rates of TGR5 and mortalin protein 75 in cancer tissues were significantly higher than those in adjacent tissues(P<0.05).WB results showed that the protein expression levels of TGR5 and mortalin protein 75 in cancer tissues were significantly higher than those in adjacent tissues(P<0.05).The expression of TGR5 protein in cancer tissues of ICC patients was correlated with gender,tumor diameter,degree of differentiation,TNM staging,satellite focus,and liver cirrhosis(P<0.05).The expression of mortalin protein 75 was correlated with tumor diameter,TNM staging,nerve involvement,satellite focus,and liver cirrhosis(P<0.05).There were significant differences in gender,tumor diameter,TNM staging,microvascular invasion,satellite focus,liver cirrhosis,and the expressions of TGR5 and mortalin protein 75 between the poor prognosis group and the good prognosis group(P<0.05).The cumulative 3-year overall survival rate of TGR5 positive patients(32.00%)was significantly lower than that of TGR5 negative patients(63.16%)

关 键 词：肝内胆管癌 胆汁酸G蛋白偶联受体5 热休克蛋白75 预后 

分 类 号：R735.8[医药卫生—肿瘤]