积雪草挥发油的神经保护作用及其化学成分的研究  

Neuroprotection related activities and chemical constituents of Centella asiatica essential oil

作　　者：梁金月 李亚娟 刘亚月 聂影影 杨志友 张永平 千忠吉[2] 林文辉 宋采 张翼 LIANG Jinyue;LI Yajuan;LIU Yayue;NIE Yingying;YANG Zhiyou;ZHANG Yongping;QIAN Zhongji;LIN Wenhui;SNG Cai;ZHANG Yi(Research Institute for Marine Drugs and Nutritions of Guangdong Ocean University,Zhanjiang Municipal Key Laboratory of Brain Health Related Marine Drugs and Nutritions,Guangdong Provincial Engineering Laboratory of Marine Biological Products,Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety,College of Food Science and Technology,Guangdong Ocean University,Zhanjiang,Guangdong,524088 P.R.China;Shenzhen Institute of Guangdong Ocean University,Shenzhen.Guangdong,524088 P.R.China;Yunfu Food and Drug Inspection Intitute,Yunfu.Guangdong,521300 P.R.China;Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University,Dalian,Liaoning,116034 P.R.China;Guangdong Tongde Pharmaceutical Co.,Ltd,Zhanjiang.Guangdong,524088 P.R.China;Lingnan Medicinal Plant Oil Branch,National Engineering Research Center for Modernization of Traditional Chinese Medicine Zhanjian,Guangdong,524088 P.R.China)

机构地区：[1]广东海洋大学食品科技学院广东省水产品加工与安全重点实验室广东省海洋生物制品工程实验室,湛江市脑健康海洋药物与营养品重点实验室广东海洋大学海洋药物研究所,广东湛江524088 [2]广东海洋大学深圳研究院,广东深圳518120 [3]云浮市食品药品检验所,广东云浮527300 [4]大连工业大学海洋食品精深加工关键技术省部共建协同创新中心,辽宁大连116034 [5]广东同德药业有限公司,广东湛江524088 [6]国家中药现代化工程技术研究中心岭南药用芳香油分中心,广东湛江524088

出　　处：《华西药学杂志》2022年第1期53-57,共5页West China Journal of Pharmaceutical Sciences

基　　金：广东省应用科技研发重大专项(2016B020235001);广东省促进经济发展专项资金项目(粤自然资源合[2019]015号);深圳市科创委基础研究项目(JCYJ20190813105005619);深圳市大鹏新区科技研发项目(KJYF202001-07);广东省普通高校重点领域专项(生物医药与健康)(2021ZDZX2064);广东省科技专项资金基础与应用基础研究专题(2021A05240);湛江市海洋经济创新发展示范市建设项目(湛海创XM-202008-01B1)。

摘　　要：目的研究积雪草挥发油的神经保护活性及其化学组成。方法采用DPPH自由基清除法和Ellman法分别评价了积雪草挥发油的抗氧化和乙酰胆碱酯酶抑制活性,采用噻唑蓝比色法测定其对β-淀粉样蛋白(Aβ)和1-甲基-4-苯基-吡啶离子(MPP^(+))诱导的SH-SY5Y神经细胞损伤的影响,采用气质联用(GC-MS)技术分析其成分。结果积雪草挥发油具有较强的抗氧化和乙酰胆碱酯酶抑制活性,对SH-SY5Y细胞有明显的促生长作用,对Aβ和MPP^(+)诱导的SH-SY5Y神经细胞损伤有显著的改善作用。积雪草挥发油中含有圆柚酮、亚油酸甲酯、角鲨烯和γ-生育酚等成分。结论积雪草挥发油在治疗神经退行性疾病方面具有研究价值。OBJECTIVE To investigate the neuroprotection related activities and the chemical constituents of Centella asiatica essential oil(CAEO).METHODS Antioxidant and acetylcholinesterase inhibitory activities of CAEO were evaluated using DPPH method and Ellman method,respectively.Effects on SH-SY5 Y cells injured by Aβand MPP;were assessed using methyl thiazolyl tetrazolium colorimetric method.Gas chromatography-mass spectroscopy(GC-MS)was used to investigate its components.RESUITS The results exhibited that CAEO showed strong antioxidant activity and acetylcholinesterase inhibitory activity.It also stimulated the growth of SH-SY5 Y cells and showed significant ameliorative effect on the injury of cultured SH-SY5 Y cells induced by Aβand MPP;.CAEO contains nootkatone,linoleic acid methyl ester,squalene andγ-tocopherol,and soon.CONCLUSION CAEO is valuable to be studied and developed on the aspect of neurodegenerative diseases’treatment.

关 键 词：积雪草 挥发油 抗氧化 乙酰胆碱酯酶抑制 神经保护 气质联用 化学组成 阿尔茨海默氏症 帕金森氏症 

分 类 号：R284[医药卫生—中药学] R96[医药卫生—中医学]