利用粪肠球菌感染根管模型评价不同根管封闭剂的抗菌效果  

To evaluate the antibacterial ability of different root canal sealers by using the Enterococcus faecalis infected root canal model

作　　者：李克朋 程甜甜 仇珺[2] 尤苏霞 蒋文凯[2] 梅笑寒 LI Kepeng;CHENG Tiantian;QIU Jun;YOU Suxia;JIANG Wenkai;MEI Xiaohan(Department of Stomatology,The PLA Navy An-Qing Hospital,Anqing 246000,China;不详)

机构地区：[1]中国人民解放军海军安庆医院口腔科,安徽安庆246000 [2]军事口腔医学国家重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔医学重点实验室,第四军医大学口腔医学院牙体牙髓病科,陕西西安710032

出　　处：《口腔医学》2022年第1期42-46,共5页Stomatology

基　　金：国家自然科学基金(81700951,81803186);创新人才推进计划-青年科技新星项目(2019KJXX-086);陕西省重点研发计划(2020SF-019)。

摘　　要：目的体外建立粪肠球菌根管感染模型,比较3种生物陶瓷类根管封闭剂的抗菌性能。方法选择人单直根管的离体前牙48颗,接种粪肠球菌并孵育4周以构建体外根管感染模型。完成根管成形和清理后,随机将样本分为3个实验组和2个对照组,每组按照如下方法进行根管充填:A组,Biodentine+牙胶;B组,iRoot BP+牙胶;C组,MTA+牙胶;D组,阳性对照组(单纯牙胶充填);E组,阴性对照组(无菌根管)。分别于第1、3、7、14天取样,利用涡轮机磨除充填材料和牙本质,收集粉末,梯度稀释后,常规进行细菌培养并计数。结果3个实验组在4个时间点均表现出对粪肠球菌的抗菌性,与阴性及阳性对照组相比,有统计学意义(P<0.05),3个实验组抗菌效果组间无明显差异(P>0.05)。此外,随时间延长3种材料杀菌效果逐渐增强,第14天达到最高值。结论Biodentine、iRoot BP、MTA具有相似的抗菌能力,均能有效杀灭粪肠球菌。Objective To establish the model of Enterococcus faecalis root canal infection in vitro and to compare the antibacterial properties of three bio-ceramic root canal sealers.Methods Forty-eight isolated anterior teeth of human single straight root canal were inoculated with Enterococcus faecalis and incubated for 4 weeks to construct an in vitro root canal infection model.After shaping and cleaning,samples were randomly divided into three experimental groups and two control groups.Each group was filled according to the following methods:A,Biodentine;B,iRoot BP;C,MTA;D,positive control group(simple cone filling);E,negative control group(sterile root canal).Samples were taken on day 1,3,7 and 14 respectively,and the powder after grinding off the filling material and dentin was collected.After gradient dilution,bacteria were cultured and counted.Results Compared with the positive control group,three experimental groups showed antibacterial activity against Enterococcus faecalis during four time points,which was statistically significant(P<0.05),and there was no significant difference among three experimental groups(P>0.05).In addition,germicidal efficacy of the three materials increased gradually with the extension of time,and reached the highest value in the 14;day.Conclusion Biodentine,iRoot BP and MTA have similar antibacterial properties and can effectively kill Enterococcus faecalis.

关 键 词：根管封闭剂 抗菌效果 粪肠球菌 感染根管模型 

分 类 号：R783.1[医药卫生—口腔医学]