尿液外泌体miR-106a联合miR-373诊断前列腺癌的价值  

作　　者：唐鹏[1] 沈利杨 张哲伟[2] 王尔平[1] 尤小明[1] 叶铿 

机构地区：[1]杭州市余杭区第一人民医院,311100 [2]浙江大学医学院附属第二医院,310009

出　　处：《浙江临床医学》2022年第2期273-275,278,共4页Zhejiang Clinical Medical Journal

摘　　要：目的探讨尿液外泌体微小RNA-106a(miR-106a)联合微小RNA-373(miR-373)诊断前列腺癌的应用价值。方法选取2016年11月至2018年4月在本院就诊的前列腺癌患者69例作为观察组,另选取同期在本院进行常规体检的健康男性69例为对照组。采用实时荧光定量PCR(qRT-PCR)检测尿液外泌体中miR-106a、miR-373的表达情况。分析miR-106a、miR-373表达与前列腺癌临床病理特征的关系。分别以miR-106a、miR-373水平为检验变量绘制受试者工作特性曲线(ROC),采用四表格分析血清前列腺特异性抗原(PSA)以及miR-106a联合miR-373检测诊断前列腺癌的应用价值,结果观察组miR-106a、miR-373的表达均明显低于对照组,差异有统计学意义(P<0.05)。前列腺癌患者尿液外泌体中miR-106a、miR-373的表达水平与临床分期、淋巴结转移和远处转移有关(P<0.05)。血清PSA诊断前列腺癌的灵敏度为94.2%,特异度为26.1%。前列腺癌患者尿液外泌体中miR-106a、miR-373水平ROC曲线的AUC分别为0.852、0.821,灵敏度分别为82.6%、72.5%,特异度分别为72.5%、78.3%。miR-106a联合miR-373诊断前列腺癌的灵敏度为92.8%,特异度为72.5%。结论miR-106a联合miR-373检测诊断前列腺癌的灵敏度、特异度均较好,具有一定的临床应用价值。Objective To investigate the application value of urinary exosomes microRNA-106a(miR-106a)combined with microRNA-373(miR-373)in the diagnosis of prostate cancer.Methods A total of 69 prostate cancer patients treated in our hospital from November 2016 to April 2018 were selected as the observation group,and 69 healthy men who underwent routine physical examination in our hospital during the same period were selected as the control group.Quantitative real-time PCR(qRT-PCR)was used to detect the expressions of miR-106a and miR-373 in urinary exosomes,the relationships between the expressions of miR-106a and miR-373 and the clinicopathological characteristics of prostate cancer were analyzed,the receiver operating characteristic curve(ROC)was drawn with the levels of miR-106a and miR-373 as test variables,and the application value of serum prostate-specific antigen(PS A)and combined detection of miR-106a and miR-373 in the diagnosis of prostate cancer were analyzed by four-grid table.Results The expressions of miR-106a and miR-373 in the experimental group were significantly lower than those in the control group,and the difference was statistically significant(P<0.05).The expression levels of miR-106a and miR-373 in urinary exosomes of prostate cancer patients were correlated with clinical stage,lymph node metastasis and distant metastasis(P<0.05).The sensitivity and specificity of semm PSA in diagnosis of prostate cancer were 94.2% and 26.1%.The ROC curves of the levels of miR-106a and miR-373 in the urine of prostate cancer patients were drawn,the AUCs of the ROC curves were 0.852 and 0.821,the sensitivities were 82.6% and 72.5%,and the specificities were 72.5% and 78.3%,respectively.The sensitivity and specificity of miR-106a combined with miR-373 in the diagnosis of prostate cancer were 92.8% and 72.5%.Conclusion Detection of miR-106a combined with miR-373 can improve the sensitivity and specificity of prostate cancer diagnosis,which has certain clinical application value.

关 键 词：尿液外泌体 微小RNA-106a 微小RNA-373 前列腺癌 诊断价值 

分 类 号：R73[医药卫生—肿瘤]