胰腺癌中小肠细菌过度生长及炎症介质异常表达分析  

Analysis between small intestinal bacteria overgrowth and abnormal expression of inflammatory mediators in pancreatic cancer

作　　者：马晓莹[1] 张鹏[2] 唐东 许琳[1] 田字彬[2] MA Xiao-ying;ZHANG Peng;TANG Dong;XU Lin;TIAN Zi-bin(Gastroenterology,Qingdao University School of Medicine,Qingdao Municipal Hospital,Qingdao,Shandong 266011,China;不详)

机构地区：[1]青岛大学附属青岛市市立医院,山东青岛266011 [2]青岛大学附属医院,山东青岛266003

出　　处：《中国微生态学杂志》2022年第1期52-56,61,共6页Chinese Journal of Microecology

基　　金：青岛市医疗卫生优秀人才培养项目(DTR2019Y)。

摘　　要：目的本研究通过检测胰腺癌患者小肠细菌过度生长(SIBO)、癌组织中IL-1β、IL-6和TNF-α的表达水平,探讨胰腺癌患者是否更易发生SIBO及SIBO伴随的炎症介质水平变化。方法采用葡萄糖氢呼气试验(HBT)检测40例胰腺癌患者和40例健康查体人群的SIBO的发生情况。采用免疫组织化学技术(IHC)测定胰腺癌组织中IL-1β、IL-6和TNF-α的表达情况,分析与胰腺癌临床病理的关系。结果胰腺癌组SIBO阳性率为60.0%,健康对照组SIBO阳性率为15.0%,胰腺癌组SIBO阳性率显著高于对照组(P<0.001)。在胰腺癌患者SIBO阳性组和阴性组间IL-1β、IL-6、TNF-α表达水平有显著差异(P<0.05)。胰腺癌患者癌组织中IL-1β的表达与分化程度及临床分期差异具有统计学意义(P<0.05)。IL-6的表达在淋巴结是否转移及年龄的差异具有统计学意义(P<0.05)。TNF-α的表达在分化程度及淋巴结是否转移的分布差异具有统计学意义(P<0.05)。结论胰腺癌患者较健康人更容易发生SIBO,SIBO可能通过促进IL-1β、IL-6及TNF-α的表达影响胰腺癌的发生发展。Objective To explore whether patients with pancreatic cancer(PC)are more prone to small intestinal bacterial overgrowth(SIBO),and analyze the changes of SIBO and inflammatory mediator levels.Methods The occurrence of SIBO was detected by using hydrogen breath test(HBT)in 40 patients with PC and 40 healthy physical examinees.The expressions of IL-1β,IL-6 and TNF-αin the PC tissue were determined with immunohistochemistry(IHC).The correlation between the clinical pathology of PC and SIBO was analyzed.Results The positive rate of SIBO was 60%in the patients with PC vs 15.0%in the healthy controls,with significant difference(P<0.001).The expression levels of IL-1β,IL-6 and TNF-αwere significantly different between the SIBO positive group and the SIBO negative group(P<0.05).The expression of IL-1βin PC tissue was significantly correlated with differentiation degree and clinical stage(P<0.05).The expression of IL-6 was significantly correlated with age and lymph node metastasis(P<0.05).TNF-αexpression was significantly correlated with lymph node metastasis and differentiation(P<0.05).Conclusion Patients with pancreatic cancer were more likely to develop SIBO than healthy people.SIBO may influence the development of pancreatic cancer by promoting the expressions of IL-1β,IL-6 and TNF-α.

关 键 词：胰腺癌 小肠细菌过度生长 炎症介质 

分 类 号：R576[医药卫生—消化系统]