结直肠癌中差异性表达的外泌体lncRNA功能富集及通路分析  

Functional enrichment and pathway analysis of differential expression of exosome lncRNA in colorectal cancer

作　　者：曾薇[1,2] 刘翼 李文婷 朱金峰 ZENG Wei;LIU Yi;LI Wen-ting;ZHU Jin-feng(First Department of Lung Cancer Chemotherapy,the Affiliated Cancer Hospital of Xinjiang Medical University,Urumqi,Xinjiang 830011,China;不详)

机构地区：[1]新疆医科大学附属肿瘤医院肺内科,新疆乌鲁木齐830011 [2]深圳大学总医院血液肿瘤科,广东深圳518055 [3]深圳大学总医院胸心外科 [4]深圳大学总医院病理科 [5]新疆医科大学附属肿瘤医院胃肠外科 [6]深圳大学总医院普外科

出　　处：《中华全科医学》2022年第1期141-143,164,共4页Chinese Journal of General Practice

基　　金：新疆维吾尔自治区自然科学基金面上项目(2018D01C255)。

摘　　要：目的通过探讨差异性表达的外泌体lncRNA及其调控的信号通路在结直肠癌发生、发展中的作用,以期为结直肠癌的诊断和治疗提供新的靶点。方法使用R软件线性回归模型软件包limma包进行差异表达分析,根据筛选阈值得到显著差异表达的lncRNA和mRNA;通过计算差异表达lncRNA与mRNA皮尔逊相关性系数,获得其之间协同表达关系,通过相关系数筛选存在正向协同表达的lncRNA-mRNA关系对;分别对lncRNA的协同表达mRNA进行GO功能和KEGG通路富集分析,以间接预测lncRNA的功能。结果在32例健康志愿者和12例结直肠癌患者中筛选出差异性表达的外泌体lncRNA共202个、mRNA共1014个。验证出差异性表达的lncRNA-mRNA关系对10个。对lncRNA协同表达的mRNA进行功能富集显示,差异性表达的外泌体lncRNA可能与B细胞受体信号通路、NF-κB信号通路、JAK-STAT信号通路、抗原处理与呈递信号通路和NOD样受体信号通路活化密切相关。结论深入探索外泌体lncRNA在结直肠癌中的差异性表达、功能富集及通路活化,有助于为结直肠癌的治疗提供新思路,为诊断提供新靶点。Objective To explore the role of differential expression of exosome lncRNA and its regulated signal pathway in the occurrence and development of colorectal cancer and provide a new target for the diagnosis and treatment of colorectal cancer.Methods The linear regression model package limma of R software was used to analyse the differential expression of lncRNA and mRNA.By calculating the Pearson correlation coefficient between differentially expressed lncRNA and mRNA,their co-expression was obtained.The positive co-expression of lncRNA-mRNA was screened by using a correlation coefficient.Go function and KEGG pathway enrichment of mRNA co-expressed with lncRNA were analysed to indirectly predict the function of lncRNA.Results A total of 202 differentially expressed exosome lncRNAs and 1014 exosome mRNA were screened from 32 healthy volunteers and 12 colorectal cancer patients.Ten pairs of lncRNA-mRNA co-expression were verified.Functional enrichment of mRNA co-expressed by lncRNA showed that differentially expressed exosome lncRNA may be closely related to B cell receptor signal pathway,NF-KB signal pathway,JAK-STAT signal pathway,antigen processing and presentation signal pathway and NOD-like receptor signal pathway activation.Conclusion In-depth exploration of differential expression,functional enrichment and pathway activation of exosome lncRNA in colorectal cancer is important to provide new ideas for the diagnosis and treatment of colorectal cancer.

关 键 词：结直肠癌 外泌体 长链非编码RNA 

分 类 号：R735.3[医药卫生—肿瘤] R34[医药卫生—临床医学]