microRNA-148b对奶牛乳腺上皮细胞乳蛋白合成的影响  

Effect of microRNA-148b on Milk Protein Synthesis in Dairy Cow Mammary Epithelial Cells

作　　者：王赛赛 都成 朱云菲 贾聚晨 赵天夺 边艳杰[1] 王春梅[1] WANG Saisai;DU Cheng;ZHU Yunfei;JIA Juchen;ZHAO Tianduo;BIAN Yanjie;WANG Chunmei(The Key Laboratory of Dairy Science of Education Ministry,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学乳品科学教育部重点实验室,哈尔滨150030

出　　处：《中国乳品工业》2022年第1期4-7,共4页China Dairy Industry

基　　金：国家自然科学青年项目(31701118)。

摘　　要：以奶牛乳腺上皮细胞(Dairy cow mammary epithelial cells,DCMECs)为研究模型,探讨microRNA-148b对DCMECs增殖和β-酪蛋白合成的影响。应用脂质体转染技术将miR-148b转染DCMECs,qRT-PCR观测其表达量,采用双荧光素酶实验验证miR-148b与转化生长因子B2(TGFB2)基因的靶向关系,分别应用EdU实验、酶联免疫吸附实验检测DCMECs的增殖以及β-酪蛋白含量。双荧光素酶实验结果证明TGFB2为miR-148b的靶基因,EdU实验结果显示,miR-148b可以促进DCMECs的增殖,ELISA结果表明过表达miR-148b可以促进β-酪蛋白合成。Using Dairy cow mammary epithelial cells(DCMECs)as a research model to explore the effects of microRNA-148b on the proliferation andβ-casein synthesis in DCMECs.Liposomal transfection technology was used to transfect miR-148b into DCMECs,qRTPCR was used to test expression of miR-148b in DCMECs.The dual luciferase experiment verified the relationship between miR-148b and TGFB2 gene.The proliferation andβ-casein content of DCMECs were detected by EdU and enzyme-linked immunosorbent assay.The result of dual luciferase experiment showed that miR-148b has a targeting relationship with TGFB2 gene.The result of EdU experiment showed that miR-148b can promote the proliferation of DCMECs,and the result of ELISA assay showed that overexpression of miR-148b can promoteβ-casein synthesis.

关 键 词：miR-148b 奶牛乳腺上皮细胞 增殖 Β-酪蛋白 

分 类 号：S823[农业科学—畜牧学]