Effect of Bacillus subtilis,Enterococcus faecium,and Enterococcus faecalis supernatants on serotonin transporter expression in cells and tissues  

作　　者：Yi-Ming Chen Ying Li Xin Wang Ze-Lan Wang Jun-Jie Hou Shuai Su Wei-Long Zhong Xin Xu Jie Zhang Bang-Mao Wang Yu-Ming Wang 

机构地区：[1]Department of Gastroenterology and Hepatology,Tianjin Medical University General Hospital,Tianjin 300052,China

出　　处：《World Journal of Gastroenterology》2022年第5期532-546,共15页世界胃肠病学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China,No.81570489;and the Youth Project of National Natural Science Foundation of China,No.81900487.

摘　　要：BACKGROUND Bacillus subtilis(B.subtilis),Enterococcus faecium(E.faecium),and Enterococcus faecalis(E.faecalis)are probiotics that are widely used in the clinical treatment of irritable bowel syndrome(IBS).Whether the supernatants of these three probiotics can improve gastrointestinal sensation and movement by regulating the serotonin transporter(SERT)expression needs to be clarified.AIM To investigate whether B.subtilis,E.faecium,and E.faecalis supernatants can upregulate SERT expression in vitro and in vivo.METHODS Caco-2 and HT-29 cells were stimulated with probiotic culture supernatants for 12 and 24 h,respectively.A male Sprague-Dawley rat model of post-infectious irritable bowel syndrome(PI-IBS)was established and the rats were treated with phosphate-buffered saline(group A)and three probiotics culture supernatants(groups B,C,and D)for 4 wk.The levels of SERT were detected by quantitative PCR and western blotting.RESULTS The levels of SERT at post-treatment 12 and 24 h were significantly elevated in Caco-2 cells treated with B.subtilis supernatant compared with those in the control group(aP<0.05).Those levels were markedly upregulated in Caco-2 cells stimulated with E.faecium and E.faecalis supernatants at 24 h(aP<0.05).In addition,SERT expression in groups B,C,and D was significantly higher than that in group A in the 2nd wk(aP<0.05).Increased SERT expression was only found in group D in the 3rd wk(aP<0.05).However,there was no significant difference in SERT expression between the groups in the last week(P>0.05).CONCLUSION The supernatants of B.subtilis,E.faecium,and E.faecalis can upregulate SERT expression in intestinal epithelial cells and the intestinal tissues in the rat model of PI-IBS.

关 键 词：Bacillus subtilis supernatant Enterococcus faecalis supernatant Enterococcus faecium supernatant Serotonin transporter expression Irritable bowel syndrome 

分 类 号：R574.4[医药卫生—消化系统]