Frankincense myrrh attenuates hepatocellular carcinoma by regulating tumor blood vessel development through multiple epidermal growth factor receptor-mediated signaling pathways  

作　　者：Piao Zheng Zhen Huang Dong-Chang Tong Qing Zhou Sha Tian Bo-Wei Chen Di-Min Ning Yin-Mei Guo Wen-Hao Zhu Yan Long Wei Xiao Zhe Deng Yi-Chen Lei Xue-Fei Tian 

机构地区：[1]College of Integrated Traditional Chinese and Western Medicine,Hunan University of Chinese Medicine,Changsha 410208,Hunan Province,China [2]Department of Integrated Traditional Chinese and Western Medicine,The Second Xiangya Hospital,Central South University,Changsha 410011,Hunan Province,China [3]The First Affiliated Hospital,Hunan University of Chinese Medicine,Changsha 410021,Hunan Province,China [4]Hunan Key Laboratory of Translational Research in Formulas and Zheng of Traditional Chinese Medicine,Hunan University of Chinese Medicine,Changsha 410208,Hunan Province,China [5]Institute of Integrative Medicine,Department of Integrated Traditional Chinese and Western Medicine,Xiangya Hospital,Central South University,Changsha 410008,Hunan Province,China

出　　处：《World Journal of Gastrointestinal Oncology》2022年第2期450-477,共28页世界胃肠肿瘤学杂志（英文版）（电子版）

基　　金：the National Natural Science Foundation of China,No.U20A20408(Major Program)and No.82074450(General Program);Natural Science Foundation of Hunan Province,No.2020JJ4066;Hunan Province Research and innovation projects for Postgraduates,No.CX20190541;Hunan Province"domestic firstclass cultivation discipline"Integrated Traditional Chinese and Western medicine open fund project,No.2018ZXYJH03;Hunan University Undergraduate Research Learning and Innovative Experiment Project,No.201609030114.

摘　　要：BACKGROUND In traditional Chinese medicine(TCM),frankincense and myrrh are the main components of the antitumor drug Xihuang Pill.These compounds show anticancer activity in other biological systems.However,whether frankincense and/or myrrh can inhibit the occurrence of hepatocellular carcinoma(HCC)is unknown,and the potential molecular mechanism(s)has not yet been determined.AIM To predict and determine latent anti-HCC therapeutic targets and molecular mechanisms of frankincense and myrrh in vivo.METHODS In the present study,which was based on the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(http://tcmspw.com/tcmsp.php),Universal Protein database(http://www.uniprot.org),GeneCards:The Human Gene Database(http://www.genecards.org/)and Comparative Toxicogenomics Database(http://www.ctdbase.org/),the efficacy of and mechanism by which frankincense and myrrh act as anti-HCC compounds were predicted.The core prediction targets were screened by molecular docking.In vivo,SMMC-7721 human liver cancer cells were transplanted as xenografts into nude mice to establish a subcutaneous tumor model,and two doses of frankincense plus myrrh or one dose of an EGFR inhibitor was administered to these mice continuously for 14 d.The tumors were collected and evaluated:the tumor volume and growth rate were gauged to evaluate tumor growth;hematoxylineosin staining was performed to estimate histopathological changes;immunofluorescence(IF)was performed to detect the expression of CD31,α-SMA and collagen IV;transmission electron microscopy(TEM)was conducted to observe the morphological structure of vascular cells;enzyme-linked immunosorbent assay(ELISA)was performed to measure the levels of secreted HIF-1αand TNF-α;reverse transcription-polymerase chain reaction(RT-qPCR)was performed to measure the mRNA expression of HIF-1α,TNF-α,VEGF and MMP-9;and Western blot(WB)was performed to determine the levels of proteins expressed in the EGFR-mediated PI3K/Akt and MAPK signaling pathways.RESULTS The result

关 键 词：Hepatocellular carcinoma Frankincense Myrrh Network pharmacology Tumor blood vessels Multiple signaling pathways 

分 类 号：R735.7[医药卫生—肿瘤]