黄芪多糖-胶原-海藻酸钠-丝素蛋白3D打印支架的制备及其生物相容性研究  

Study of Preparation and Biocompatibility of Astragalus Polysaccharide-Collagen-Sodium Alginate-Silk Fibroin 3D Printing Scaffolds

作　　者：杜伟斌[1] 沈福祥 杨亚冬[2] 陈荣良[1] 华爱兰 王拓[1] 曹国平[1] DU Weibin;SHEN Fuxiang;YANG Yadong;CHEN Rongliang;HUA Ailan;WANG Tuo;CAO Guoping(Department of Orthopedics,Jiangnan Hospital Affiliated to Zhejiang Chinese Medical University,Hangzhou 311201,China;Institute of Tissue Engineering,Zhejiang Province Academy of Medical Sciences,Hangzhou310013,China)

机构地区：[1]浙江中医药大学附属江南医院(杭州市萧山区中医院)骨科,杭州311201 [2]浙江省医学科学院组织工程研究所(杭州医学院)

出　　处：《中国中医骨伤科杂志》2022年第1期1-4,共4页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：国家自然科学基金青年基金(81904053);浙江省医药卫生科技计划项目(2020KY796,2020KY797);杭州市萧山区社会发展重大科技计划项目(11216,2019318)。

摘　　要：目的:应用3D打印技术制备黄芪多糖-胶原-海藻酸钠-丝素蛋白复合支架,分析其生物相容性。方法:2020年8月至2020年12月,采用MTT法分析HeLa细胞增殖抑制率,选择黄芪多糖最适工作浓度区间;制备黄芪多糖-胶原-海藻酸钠-丝素蛋白3D打印支架,进行形态学观察。通过细胞共培养显微镜观察、Calcein-AM/PI活死细胞双染、扫描电镜等比较,分析其生物相容性。结果:50μg/mL浓度黄芪多糖对细胞增殖抑制程度较低,且可促进细胞增殖。3D打印支架孔径大小一致,间距统一,厚薄均匀。细胞共培养5 d后,向支架内部迁移,呈团簇状生长。染色可见细胞生长良好,无明显的细胞死亡现象。扫描电镜下可见丰富的细胞黏附在支架上,细胞间有突触相连。结论:胶原-海藻酸钠-丝素蛋白含黄芪多糖(工作浓度25~400μg/mL)的3D打印支架性能最佳,可为创面修复、软组织退肿等医用敷料的应用提供新思路。Objective:To prepare astragalus polysaccharide-collagen-sodium alginate-silk fibroin 3 D printing scaffolds and study its biocompatibility.Methods:From August 2020 to December 2020,the proliferation inhibition rate of HeLa cells was analyzed by MTT assay to select the optimal working concentration range of APS.Astragalus polysaccharide-collagen-sodium alginate-silk fibroin 3 D printing scaffolds were prepared for morphological observation,and the biocompatibility was analyzed by cell co-culture microscopy,double staining of dead cells with Calcein-AM/PI and scanning electron microscopy.Results:Astragalus polysaccharide(50 μg/mL) had a low inhibitory efficacy on cell proliferation and promoted cell proliferation.The 3 D printing bracket had uniform aperture size,spacing and thickness.After 5 d later of co-culture,the cells migrated to the inside of the scaffold and grew in clusters.Staining showed that the cell growth was well,and there was no obvious cell death.Scanning electron microscopy showed there were abundant cells adhesion on the scaffold and synaptic connection between cells.Conclusion:The 3 D printing scaffolds with collagen-alginate-silk fibroin protein containing astragalus polysaccharide(working concentration 25 to 400 μg/mL) had the best performance,which can provide a new idea for the application of medical dressings such as wound repair and soft tissue swelling reduction.

关 键 词：黄芪多糖 胶原 海藻酸钠 丝素蛋白 支架材料 医用敷料 

分 类 号：R-3[医药卫生]