食品中志贺氏菌属的测定能力验证结果分析  

The Result Analysis of Proficiency Testing on Shigella Examination in Food

作　　者：王丽杰 何丽萍 杨晓晖 张明新 WANG Lijie;HE Liping;YANG Xiaohui;ZHANG Mingxin(Henan Zhongce Technology Testing Service Co.,Ltd.,Zhengzhou 450000,China)

机构地区：[1]河南中测技术检测服务有限公司,河南郑州450000

出　　处：《现代食品》2022年第1期151-154,共4页Modern Food

摘　　要：目的:参加编号为CFAPA-1192的食品中志贺氏菌属的测定能力验证实验,验证本检测机构检测志贺氏菌属的能力。方法:根据能力验证参试指导书,并参照GB 4789.5—2012检测志贺氏菌属能力验证样品,对分离出的可疑菌落,采用HBI志贺氏菌生化鉴定条鉴定,同时采用恒温扩增荧光检测仪法作为辅助方法。结果:样品M;中检出志贺氏菌,样品M;和M;中未检出志贺氏菌,恒温扩增荧光法与国家标准法检测结果一致。结论:本次实验室的能力验证结果为"满意",验证了本检测机构检测志贺氏菌的能力。Objective:To participate in proficiency testing of Shigella in food numbered CFAPA-1192,and verify the testing ability of Shigella in laboratory.Methods:Participate in the test instruction according to the ability verification,and the national standard method GB 4789.5—2012 was used to detect the samples of Shigella proficiency testing.The suspicious colonies were identified by biochemical identification strip of Shigella, and thermostatic amplified fluorescence method was used as an auxiliary method.Results:Shigella was detected in sample M;,while was not detected in sample M;and M;.The results of thermostatic amplified fluorescence method were consistent with the national standard method.Conclusion:The proficiency testing results of the laboratory are satisfactory,which effectively verifies the laboratory’s ability to detect Shigella.

关 键 词：志贺氏菌 鉴定 能力验证 恒温扩增荧光 

分 类 号：TS207.4[轻工技术与工程—食品科学]